Azar Y, Eyal-Giladi H
J Embryol Exp Morphol. 1979 Aug;52:79-88.
(1) Removal of both the area opaca and the marginal zone of the area pellucida from a blastoderm stripped of its hypoblast (type-IV operation) prevents the regeneration of a normally functioning primary hypoblast. (2) Stage-XIII E.G. & K blastoderms (prior to the appearance of PS) after a type-IV operation do not form a primitive streak. (3) In order type-IV operated blastoderms in which the primitive streak has already begun to appear, the regeneration of a non-functional hypoblast did not support the normal maturation of the primitive streak, and in many cases the already existing rudimentary streak was absorbed. (4) Type-IV operated blastoderms from stage 3+ H + H and on developed normally. (5) It is concluded that the cellular contribution of the marginal zone to the primary hypoblast is instrumental in the latter's capacity to induce a PS.
(1) 从去除了下胚层的胚盘上移除不透明区和透明区的边缘区域（IV型手术）会阻止正常功能的初级下胚层的再生。(2) IV型手术后的XIII期E.G. & K胚盘（原条出现之前）不会形成原条。(3) 对于已经开始出现原条的IV型手术操作的胚盘，无功能下胚层的再生不支持原条的正常成熟，并且在许多情况下，已有的原始条带会被吸收。(4) 3+H+H期及之后的IV型手术操作的胚盘发育正常。(5) 得出的结论是，边缘区域对初级下胚层的细胞贡献有助于后者诱导原条的能力。
