Vanroelen C, Verplanken P, Vakaet L C
J Embryol Exp Morphol. 1982 Aug;70:189-96.
The hypoblast of early-primitive-streak-stage chick blastoderms was partially removed. This experiment provokes a reaction in the epiblast which curls up and becomes even at its ventral surface. The basal lamina underlying the epiblast is also dependent upon the presence of hypoblast. During culture after partial hypoblast removal, active hypoblast wound healing is observed. Where the hypoblast underlies the epiblast again, the effects of the removal disappear and normal development proceeds. The results suggest that the normal epiblast morphology is dependent upon the presence of hypoblast. This influence of hypoblast on epiblast is thought to be concerned with the morphology of the epiblast and not directly with its morphogenesis.
早期原条期鸡胚胚盘的下胚层被部分移除。该实验引发上胚层的反应，上胚层卷曲，其腹面变得平整。上胚层下方的基膜也依赖于下胚层的存在。在部分下胚层移除后的培养过程中，观察到活跃的下胚层伤口愈合。当下胚层再次位于上胚层下方时，移除的影响消失，正常发育继续进行。结果表明，正常上胚层形态依赖于下胚层的存在。下胚层对上胚层的这种影响被认为与上胚层的形态有关，而不是直接与其形态发生有关。
