Bock Susan Clark, Tiemeier David Charles, Mester Katalin, Goldsmith Marian Ruth
Rockefeller University, 10021, New York, NY.
Molecular Biology Laboratories, Monsanto Company, 63166, St. Louis, MO, USA.
Wilehm Roux Arch Dev Biol. 1983 Sep;192(5):222-227. doi: 10.1007/BF00848653.
cDNA clones for two distinct families ofBombyx mori chorion protiens, Class A (m2774) and Class Hc (m5000), were used to study the developmental regulation of specific chorion mRNAs. Individual follicles from maturing ovarioles were assayed by Northern blotting techniques and amino acid pulse-labeling to determine concomitant RNA accumulation and protein synthesis patterns with a temporal resolution of 2.2-2.6 h of developmental age. RNAs hybridizing to the two cloned chorion sequences at high criterion showed distinctive, non-overlapping patterns of accumulation during the middle-late and very late stages of choriogenesis. Moreover, the periods of expression of these RNAs coincided exactly with the synthetic periods of two distinct subsets of chorion proteins, one containing Class A and B components, and the other containing Class Hc components alone. These results suggest that chorion gene transcription and translation are tightly coupled.
家蚕绒毛膜蛋白的两个不同家族，即A类（m2774）和Hc类（m5000）的cDNA克隆，被用于研究特定绒毛膜mRNA的发育调控。通过Northern印迹技术和氨基酸脉冲标记对成熟卵巢管中的单个卵泡进行检测，以确定伴随RNA积累和蛋白质合成模式，发育年龄的时间分辨率为2.2 - 2.6小时。在高严格度下与两个克隆的绒毛膜序列杂交的RNA在成膜中期和晚期显示出独特的、不重叠的积累模式。此外，这些RNA的表达时期与绒毛膜蛋白的两个不同亚组的合成时期恰好一致，一个亚组包含A类和B类成分，另一个亚组仅包含Hc类成分。这些结果表明绒毛膜基因的转录和翻译紧密偶联。
