Katow H, Hayashi M
J Cell Biol. 1985 Oct;101(4):1487-91. doi: 10.1083/jcb.101.4.1487.
We studied the effect of fibronectin (FN) on the behavior of primary mesenchyme cells isolated from sea urchin mesenchyme blastulae in vitro using a time-lapse technique. The migration of isolated primary mesenchyme cells reconstituted in seawater and horse serum is dependent on the presence or absence of exogenous FN in the culture media. The cells in FN, 4 and 40 micrograms/ml, show a high percentage of migration and migrate long distances, whereas a higher concentration of FN at 400 micrograms/ml tends to inhibit migration.
我们使用延时摄影技术,在体外研究了纤连蛋白(FN)对从海胆间充质囊胚分离出的原代间充质细胞行为的影响。在海水和马血清中重构的分离原代间充质细胞的迁移取决于培养基中外源FN的存在与否。在含有4微克/毫升和40微克/毫升FN的培养基中的细胞,迁移率很高,且能远距离迁移,而400微克/毫升的较高浓度FN往往会抑制迁移。