Biocenter, University of Basel, CH-4056 Basel, Switzerland.
EMBO J. 1987 Jun;6(6):1587-93. doi: 10.1002/j.1460-2075.1987.tb02404.x.
The extracellular matrix is involved in the regulation of differentiation and morphogenesis. Here we report the identification of a sea urchin embryonic extracellular matrix protein by means of a monoclonal antibody BL1 (Mab BL1) and the isolation of the protein from basal lamina preparations. In paraffin sections of fixed embryos, the antibody can be detected on the basal surfaces of cells after the blastula stage. Immunoprecipitation from embryo lysates and salt extracts of metabolically labeled basal lamina preparations demonstrates that the basal lamina antigen is a large mol. wt protein of approximate mol. wt 10 which consists of disulfide-linked subunits of mol. wts 480 000 and 260 000. Electron microscopic images show that the Mab BL1 basal lamina antigen is structurally related to the vertebrate extracellular matrix protein laminin.
细胞外基质参与调节分化和形态发生。在此，我们通过单克隆抗体 BL1（Mab BL1）鉴定了一种海胆胚胎细胞外基质蛋白，并从基底膜制剂中分离出该蛋白。在固定胚胎的石蜡切片中，胚泡期后，抗体可在细胞的基底表面检测到。从胚胎裂解物和代谢标记的基底膜制剂的盐提取物中进行免疫沉淀表明，基底膜抗原是一种大分子量蛋白，约为 10 分子量，由分子量为 480000 和 260000 的二硫键连接的亚单位组成。电子显微镜图像显示，Mab BL1 基底膜抗原在结构上与脊椎动物细胞外基质蛋白层粘连蛋白有关。
