Moreau Nicole, Angelier Nicole, Lautredou Nicole
Centre de Biologie Cellulaire, CNRS, 67 rue Maurice Günsbourg, F-94205, Ivry sur Seine Cedex, France.
Université Pierre et Marie Curie, 4 Place Jussieu, F-75005, Paris, France.
Rouxs Arch Dev Biol. 1990 Nov;199(3):181-187. doi: 10.1007/BF01681492.
Affinity-purified antibodies directed against an 82-kDa oocyte nuclear protein ofPleurodeles waltl (Amphibia, Urodela) were prepared using antigen bound to nitrocellulose paper. The specificity of the antibody was controlled on two-dimensional electrophoretic gels of nuclear proteins. The intranuclear distribution of the 82-kDa protein was analyzed by the indirect immunofluorescence method on spreads of oocyte nuclear content. Localization of the protein appeared to be extremely variable. The antibody recognized the protein (a) on normal and landmark loop matrices (but not simultaneously), (b) on ribonucleoprotein particles associated (or not) with the nucleoskeleton and (c) on nucleoli. This suggests the intervention of the protein at a certain physiological moment in the transcriptional or posttranscriptional process.
使用与硝酸纤维素纸结合的抗原制备了针对无斑雨蛙（两栖纲，有尾目）82 kDa卵母细胞核蛋白的亲和纯化抗体。在核蛋白的二维电泳凝胶上检测了抗体的特异性。通过间接免疫荧光法对卵母细胞核内容物涂片进行分析，研究了82 kDa蛋白在核内的分布。该蛋白的定位似乎极具变异性。该抗体在（a）正常和标志性环基质上（但不是同时）、（b）与核骨架相关（或不相关）的核糖核蛋白颗粒上以及（c）核仁上识别该蛋白。这表明该蛋白在转录或转录后过程的特定生理时刻发挥作用。
