Olmsted J B
J Biol Chem. 1981 Dec 10;256(23):11955-7.
A method is described for the affinity purification of antibodies using protein samples that have been electrophoretically transferred to diazotized paper. Using differentiated neuroblastoma cells as the protein sample and a heterogeneous anti-microtubule protein serum, antibodies were isolated that specifically bound only to tubulin on blots and that stained microtubule networks in cells. The general utility of this method for various types of applications is discussed.
本文描述了一种利用已通过电泳转移至重氮化纸上的蛋白质样品进行抗体亲和纯化的方法。以分化的神经母细胞瘤细胞作为蛋白质样品，并使用异种抗微管蛋白血清，分离出了仅在印迹上特异性结合微管蛋白且能对细胞中的微管网络进行染色的抗体。本文还讨论了该方法在各类应用中的通用性。
