Pampfer Serge, Vanderheyden Ivo, Michiels Bénédicte, De Hertogh René
Physiology of Human Reproduction Research Unit, OBST 5330, University of Louvain, 53 Avenue Mounier, B-1200, Brussels, Belgium.
Rouxs Arch Dev Biol. 1990 Feb;198(5):257-263. doi: 10.1007/BF00377392.
The number of trophectoderm (TE) and inner cell mass (ICM) cells was determined by complementmediated lysis and differential staining in rat embryos collected at different times during in vivo preimplantation development. At 90 h after fertilization, two groups of morulae were discriminated according to the presence or absence of detectable ICM cells, and the analysis of their total cell number indicated that acquisition of a permeability seal between TE cells begins at the 14-cell stage. On the other hand, our data confirmed that blastocoele formation occurs after the fourth cleavage division in the rat. The total cell number increased exponentially with time in blastocysts recovered between 90 h and 127 h but the cell kinetics of TE and ICM cells were different. The proportion of ICM cells consequently varied throughout blastocyst development, with a peak value for expanded blastocysts at 103 h. Finally, a linear-quadratic relationship was found between the numbers of TE and ICM cells when all the embryos with a detectable ICM were analysed together.
在体内植入前发育的不同时间收集大鼠胚胎，通过补体介导的细胞裂解和差异染色来确定滋养外胚层（TE）和内细胞团（ICM）细胞的数量。受精后90小时，根据是否存在可检测到的ICM细胞，区分出两组桑葚胚，对其总细胞数的分析表明，TE细胞之间通透性封闭的形成始于14细胞阶段。另一方面，我们的数据证实，大鼠在第四次卵裂后发生囊胚腔形成。在90小时至127小时之间回收的囊胚中，总细胞数随时间呈指数增加，但TE和ICM细胞的细胞动力学不同。因此，ICM细胞的比例在整个囊胚发育过程中有所变化，在103小时时扩张囊胚的比例达到峰值。最后，当对所有具有可检测到ICM的胚胎进行综合分析时，发现TE和ICM细胞数量之间存在线性二次关系。
