Frey Anne, Gutzeit Herwig
Institut für Biologie I (Zoologie), Universität Freiburg, Albertstraße 21a, D-7800, Freiburg, Germany.
Rouxs Arch Dev Biol. 1986 Oct;195(8):527-531. doi: 10.1007/BF00375894.
In aberrant egg follicles of the pattern mutant dicephalic (dic) the oocyte is wedged in between two groups of nurse cells, and this condition may give rise to embryos which express anterior traits at both ends. We have analysed the role of the dic genotype of the germ line cells and the surrounding somatic follicle cells in the formation of the dic follicular phenotype. By means of pole cell transplantations into Fs (1) K 1237 hosts (this cell-autonomous mutation causes degeneration of the host's germ line cells early in oogenesis), we constructed chimeras in which either the follicle cells, the germ line cells, or both were homozygous for the dic mutation. In all three combinations the dic phenotype was expressed but not in controls with dic in both germ line cells and follicular epithelium. Since follicles with the dic phenotype may be produced if either the germ line cells or the follicle cells lack dic gene activity we suggest that cellular interactions between both cell types are required for the correct positioning of the oocyte at the follicle's posterior pole.
在模式突变体双头（dic）的异常卵泡中，卵母细胞被夹在两组滋养细胞之间，这种情况可能会产生两端都表达前部特征的胚胎。我们分析了生殖系细胞和周围体细胞卵泡细胞的dic基因型在dic卵泡表型形成中的作用。通过将极细胞移植到Fs(1)K 1237宿主中（这种细胞自主突变会导致宿主生殖系细胞在卵子发生早期退化），我们构建了嵌合体，其中卵泡细胞、生殖系细胞或两者对于dic突变都是纯合的。在所有三种组合中都表达了dic表型，但在生殖系细胞和卵泡上皮细胞中都有dic的对照中则没有表达。由于如果生殖系细胞或卵泡细胞缺乏dic基因活性，就可能产生具有dic表型的卵泡，我们认为两种细胞类型之间的细胞相互作用对于卵母细胞在卵泡后极的正确定位是必需的。
