Lawrence P A, Johnston P, Struhl G
Cell. 1983 Nov;35(1):27-34. doi: 10.1016/0092-8674(83)90204-0.
Some homeotic genes of Drosophila are active only in particular developmental compartments ("selector" genes) whereas others are active in all compartments and are responsible for regulating selector gene expression. Using pole cell transplants to generate mosaic females with mutant germ lines, we examine the extent to which genes of both classes are required during oogenesis. We find that none of four selector genes (Ultrabithorax+, Antennapedia+, Sex combs reduced+, and engrailed+) is required for the development of viable eggs, and none contributes detectable product to the fertilized zygote. Similarly, neither of two regulatory genes (Polycomb+ and extra sex combs+) are required for normal oogenesis. However, both are active in the germ line and contribute functional gene product to the embryo. These results suggest that selector genes are inactive in the germ line where they may be regulated by a different mechanism than in the soma.
果蝇的一些同源异型基因仅在特定的发育区室中活跃（“选择器”基因），而其他基因在所有区室中都活跃，并负责调节选择器基因的表达。我们利用极细胞移植来产生具有突变种系的嵌合雌性，以此研究在卵子发生过程中这两类基因的必需程度。我们发现，四个选择器基因（超双胸 +、触角足 +、性梳减少 + 和 engrailed +）中没有一个对于可存活卵子的发育是必需的，并且没有一个会向受精卵贡献可检测到的产物。同样，两个调控基因（多梳 + 和额外性梳 +）对于正常的卵子发生也不是必需的。然而，这两个基因在种系中都活跃，并向胚胎贡献功能性基因产物。这些结果表明，选择器基因在种系中是不活跃的，在种系中它们可能受与体细胞中不同的机制调控。
