Matsushita Susumu, Matsushita S
Department of Biology, Tokyo Women's Medical College, Shinjuku-ku, 162, Tokyo, Japan.
Rouxs Arch Dev Biol. 1995 Jun;204(6):392-399. doi: 10.1007/BF00360484.
Various portions of the splanchnopleural mesoderm lateral to the somites of 1.5-day chick embryos were marked in ovo by local injection of Dil, and the distribution of the labelled cells in the digestive-tract mesoderm formed after 3 days' reincubation was analysed. The presumptive area of the digestive organs was confined to bands of splanchnic mesoderm lying lateral to the somites, on both sides, with a width two or three times that between the midline of the embryo and the lateral edge of the somite. Each band generally contributed cells to its own side of the digestive-tract mesoderm, except for the region around the bile duct. The anterior and posterior portion of the pre-gut area contributed cells to the anterior and posterior region of the digestive tract, respectively, but label originating from the portion furthest from the somite took the more ventral and posterior position. Thus, the presumptive areas of the respective digestive organs were located anteroposteriorly in the same order as in the digestive tract with their boundaries lying oblique to the embryonic axis.
通过在1.5日龄鸡胚体节外侧的脏壁中胚层不同部位局部注射Dil进行胚内标记，然后分析再孵育3天后形成的消化道中胚层中标记细胞的分布。消化器官的预定区域局限于体节两侧外侧的脏壁中胚层带，其宽度是胚胎中线与体节外侧边缘之间宽度的两到三倍。除了胆管周围区域外，每条带通常为消化道中胚层自身一侧提供细胞。前肠区域的前部和后部细胞分别贡献给消化道的前部和后部区域，但来自离体节最远部分的标记占据更腹侧和后侧的位置。因此，各个消化器官的预定区域在前后方向上的排列顺序与消化道相同，其边界与胚胎轴呈斜交。
