Bottke Werner, Burschyk Monika, Volmer Jutta
Zoologisches Institut der Universität, Schlossplatz 5, D-4400, Münster, Federal Republic of Germany.
Rouxs Arch Dev Biol. 1988 Dec;197(7):377-382. doi: 10.1007/BF00398988.
The iron storage protein, ferritin, is the major yolk protein in freshwater snails. In this report we show by in vitro labelling experiments that yolk ferritin of the snails Lymnaea stagnalis L. and Planorbarius corneus L. is an exogenous protein synthesized in the midgut gland and secreted into the hemolymph. Gonad and mantle tissue are inactive in the synthesis of yolk ferritin, but, together with the midgut gland, they synthesize another ferritin type (soma ferritin) which is not released into the hemolymph and which may be a housekeeping ferritin. Soma ferritin and yolk ferritin are not in a precursor/product relationship since subunits of both ferritins are synthesized as primary translation products in rabbit reticulocyte lysate programmed with poly (A) RNA from midgut gland and gonad. Results suggest that both ferritins are synthesized on different mRNAs (and possibly on different genes) so they may be regulated in a different way.
铁储存蛋白——铁蛋白，是淡水螺类的主要卵黄蛋白。在本报告中，我们通过体外标记实验表明，椎实螺和角螺的卵黄铁蛋白是一种外源性蛋白，它在中肠腺中合成并分泌到血淋巴中。性腺和外套膜组织在卵黄铁蛋白的合成中不活跃，但它们与中肠腺一起合成另一种铁蛋白类型（体细胞铁蛋白），这种铁蛋白不会释放到血淋巴中，可能是一种管家铁蛋白。体细胞铁蛋白和卵黄铁蛋白不存在前体/产物关系，因为这两种铁蛋白的亚基都是在用来自中肠腺和性腺的聚（A）RNA编程的兔网织红细胞裂解物中作为初级翻译产物合成的。结果表明，这两种铁蛋白是在不同的mRNA上（可能在不同的基因上）合成的，因此它们可能以不同的方式受到调控。
