Melton D A
Proc Natl Acad Sci U S A. 1985 Jan;82(1):144-8. doi: 10.1073/pnas.82.1.144.
As a test for a method to analyze gene function in embryogenesis, the translation of a specific mRNA has been blocked in vivo by microinjection of complementary (anti-sense) RNA. RNA complementary to globin mRNA was synthesized in vitro by transcription of an inverted globin cDNA clone. After injection into frog oocyte cytoplasm, the anti-sense globin RNA forms a hybrid with globin mRNA and selectively prevents its translation. Deletion mapping of the anti-sense RNA shows that the 5' region of the globin mRNA must be covered in order to block translation. This method may allow one to study the function of many genes for which DNA clones are available by preventing the expression of the endogenous gene as protein.
作为一种分析胚胎发育中基因功能方法的测试，通过显微注射互补（反义）RNA在体内阻断了特定mRNA的翻译。通过对反向珠蛋白cDNA克隆进行转录，在体外合成了与珠蛋白mRNA互补的RNA。将其注射到蛙卵母细胞细胞质中后，反义珠蛋白RNA与珠蛋白mRNA形成杂交体，并选择性地阻止其翻译。反义RNA的缺失图谱显示，为了阻断翻译，珠蛋白mRNA的5'区域必须被覆盖。通过阻止内源性基因作为蛋白质表达，该方法可能使人们能够研究许多有DNA克隆可用的基因的功能。
