Hatakeyama Masatsugu, Sawa Masami, Oishi Kugao
Department of Biology, Faculty of Science, Kobe University, 657, Nada, Kobe, Japan.
Department of Biology, Aichi University of Education, 448, Kariya, Aichi, Japan.
Rouxs Arch Dev Biol. 1994 Aug;203(7-8):450-453. doi: 10.1007/BF00188695.
Mature unfertilized eggs (oocytes) dissected from the ovary of the sawfly Athalia rosae (Hymenoptera) begin parthenogenetic development if exposed to distilled water and produce haploid males. Injection of sperm into mature oocytes through the anterior pole resulted in karyogamy in a fraction of cases which developed as diploid females. No haploid-haploid chimeras due to independent participation of the injected sperm in development were produced. When sperm were injected through the posterior pole, however, fertilization never occurred but haploid-haploid chimeras were produced in a smaller fraction of cases. Both egg nucleus-derived and injected sperm-derived nuclei contributed in forming the germ cells of the chimeric males.
从叶蜂（膜翅目）卵巢中解剖出的成熟未受精卵（卵母细胞），如果暴露于蒸馏水中，会开始孤雌生殖发育并产生单倍体雄性。通过前极将精子注入成熟卵母细胞，在部分情况下会发生核融合，发育为二倍体雌性。未产生因注入的精子独立参与发育而形成的单倍体 - 单倍体嵌合体。然而，当通过后极注入精子时，受精从未发生，但在较小比例的情况下会产生单倍体 - 单倍体嵌合体。卵细胞核来源的和注入精子来源的细胞核都参与形成嵌合体雄性的生殖细胞。
