Hatakeyama Masatsugu, Nakamura Tetsuya, Kim Kyu Beom, Sawa Masami, Naito Tikahiko, Oishi Kugao
Department of Biology, Faculty of Science, Kobe University, Nada, 657, Kobe, Japan.
Division of Science of Biological Resources, Graduate School of Science and Technology, Kobe University, Nada, 657, Kobe, Japan.
Rouxs Arch Dev Biol. 1990 May;198(7):389-394. doi: 10.1007/BF00376157.
Mature eggs dissected from ovaries of unmated females of Athalia rosae (Hymenoptera: Tenthredinidae), if placed on a filter-paper soaked with distilled water, are activated and develop to haploid males. Occasionally, however, diploid females develop from these artificially activated eggs. Treatment of mature unfertilized eggs dissected from diploid females with ice-cold temperatures immediately before activation and with a high temperature (36° C) upon and immediately after activation resulted in the production of diploid males, diploid females, triploid females and gynandromorphs at high frequency. The same treatment of mature unfertilized eggs dissected from triploid females resulted in the production of only triploid survivors. These results, together with the results on the segregation of a marker mutation, yellow fatbody (yfb), appear to indicate that meiotic divisions were complete in the treated eggs, and that all four nuclei became potentially capable of participating in development with or without automictic fusion.
从蔷薇叶蜂（膜翅目：叶蜂科）未交配雌虫卵巢中解剖出的成熟卵，如果放置在浸有蒸馏水的滤纸上，会被激活并发育为单倍体雄虫。然而，偶尔也会有二倍体雌虫从这些人工激活的卵中发育出来。在激活前立即用冰冷温度处理从二倍体雌虫解剖出的成熟未受精卵，并在激活时及激活后立即用高温（36℃）处理，结果导致高频率地产生二倍体雄虫、二倍体雌虫、三倍体雌虫和雌雄嵌合体。对从三倍体雌虫解剖出的成熟未受精卵进行同样处理，结果只产生了三倍体存活个体。这些结果，连同关于标记突变黄脂肪体（yfb）分离的结果，似乎表明在处理过的卵中减数分裂是完整的，并且所有四个细胞核无论有无自体融合都有可能参与发育。
