Tiedemann Hildegard
Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Arnimallee 22, D-14195, Berlin, Germany.
Rouxs Arch Dev Biol. 1993 Jan;203(1-2):28-33. doi: 10.1007/BF00539887.
Embryos of Ambystoma mexicanum from the late morula to the late blastula stage were dissected and cultivated in varying combinations. The marginal zone (presumptive mesoderm) when isolated together with the vegetal region differentiated to notochord after dissection from early blastulae, but did not differentiate to other tissues. When isolated from middle to late blastulae, in addition myoblasts and mesenchyme were formed. The marginal zone isolated together with the animal region (presumptive ectoderm) differentiated to notochord, muscle, mesenchyme, renal tubules and mesothelium irrespective of the stage of dissection. Combination of isolated animal and vegetal regions did lead to the induction of mesodermal organs. The experiments suggest that further steps in the differentiation of mesodermal organs after the induction of mesoderm by the vegetalizing factor depend on factors from the animal region, which are involved in pattern formation.
将墨西哥钝口螈从桑椹胚晚期到囊胚晚期的胚胎进行解剖，并以不同组合进行培养。边缘区（预定中胚层）与植物区一起分离时，从早期囊胚解剖后可分化为脊索，但不会分化为其他组织。从中期到晚期囊胚分离时，此外还会形成成肌细胞和间充质。边缘区与动物区（预定外胚层）一起分离时，无论解剖阶段如何，都会分化为脊索、肌肉、间充质、肾小管和间皮。分离的动物区和植物区的组合确实导致了中胚层器官的诱导。这些实验表明，在植物化因子诱导中胚层后，中胚层器官分化的进一步步骤取决于来自动物区的因子，这些因子参与模式形成。
