Schenkel Heide, Scheller Klaus
Zoologisches Institut der Universität Würzburg, Röntgenring 10, D-8700, Würzburg, Germany.
Rouxs Arch Dev Biol. 1986 Jul;195(5):290-295. doi: 10.1007/BF00376061.
The stage- and tissue-specific biosynthesis of calliphorin was analysed during the development of the blowfly, Calliphora vicina. Western blot analyses show that the protein is not present in eggs, whereas it can be detected in fat body, brain, imaginai disk, salivary gland and epidermis throughout all postembryonic stages, including the adult one. By Northern analysis a unique 2.6 kb mol.wt. mRNA coding for calliphorin is identified exclusively in the fat body tissue of larvae, pupae and adults. Hybridization experiments of in vivo labelled poly(A) RNA with filter-bound calliphorin genes indicate that the genes are transcribed until pupariation. However, the translation of the calliphorin mRNA stops at the end of the feeding stage, as shown by [S]-methionine incorporation.
在丽蝇（Calliphora vicina）的发育过程中，对丽蝇素的阶段和组织特异性生物合成进行了分析。蛋白质免疫印迹分析表明，该蛋白在卵中不存在，而在包括成虫阶段在内的所有胚后阶段的脂肪体、脑、成虫盘、唾液腺和表皮中都能检测到。通过Northern分析，在幼虫、蛹和成虫的脂肪体组织中专门鉴定出一种编码丽蝇素的独特的2.6 kb分子量的mRNA。体内标记的多聚腺苷酸RNA与固定在滤膜上的丽蝇素基因的杂交实验表明，这些基因一直转录到化蛹期。然而，如[S]-甲硫氨酸掺入实验所示，丽蝇素mRNA的翻译在取食阶段结束时停止。
