• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

棕尾别麻蝇肌蛋白信使核糖核酸的鉴定

Identification of storage-protein messenger RNA of the fleshfly Sarcophaga peregrina.

作者信息

Tahara T, Maeda Y, Kuroiwa A, Ueno K, Obinata M, Natori S

出版信息

Biochem J. 1982 Jun 1;203(3):571-5. doi: 10.1042/bj2030571.

DOI:10.1042/bj2030571
PMID:6180730
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1158270/
Abstract

Storage-protein mRNA was found to be abundant in poly(A)-containing RNA extracted from the fat-body of third-instar larvae of Sarcophaga peregrina (fleshfly). This RNA sedimented at the position of 19S on sucrose-density-gradient centrifugation and the product of its translation in vitro was 75K protein (protein of mol.wt. 75 000), which was precipitated specifically with antibody against storage protein. This product was suggested to contain a signal sequence that is missing in mature storage protein. The poly(A)-containing RNA was also found to contain much of another mRNA coding for 25K protein (protein of mol.wt. 25 000), but the function of this protein is unknown.

摘要

在棕尾别麻蝇（肉蝇）三龄幼虫脂肪体中提取的含多聚腺苷酸（poly(A)）的RNA中，发现储存蛋白mRNA含量丰富。该RNA在蔗糖密度梯度离心中沉降于19S位置，其体外翻译产物是75K蛋白（分子量为75000的蛋白），该蛋白能被抗储存蛋白抗体特异性沉淀。推测该产物含有成熟储存蛋白中缺失的信号序列。还发现含poly(A)的RNA中含有大量另一种编码25K蛋白（分子量为25000的蛋白）的mRNA，但该蛋白的功能未知。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bad/1158270/34fa9c70da98/biochemj00376-0054-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bad/1158270/f27b35344f84/biochemj00376-0052-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bad/1158270/cb16ae8f4866/biochemj00376-0052-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bad/1158270/7eb864cdc36b/biochemj00376-0053-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bad/1158270/d373668191d8/biochemj00376-0053-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bad/1158270/34fa9c70da98/biochemj00376-0054-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bad/1158270/f27b35344f84/biochemj00376-0052-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bad/1158270/cb16ae8f4866/biochemj00376-0052-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bad/1158270/7eb864cdc36b/biochemj00376-0053-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bad/1158270/d373668191d8/biochemj00376-0053-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9bad/1158270/34fa9c70da98/biochemj00376-0054-a.jpg

相似文献

1
Identification of storage-protein messenger RNA of the fleshfly Sarcophaga peregrina.棕尾别麻蝇肌蛋白信使核糖核酸的鉴定
Biochem J. 1982 Jun 1;203(3):571-5. doi: 10.1042/bj2030571.
2
Purification of Sarcophaga (fleshfly) lectin and detection of sarcotoxins in the culture medium of NIH-Sape-4, an embryonic cell line of Sarcophaga peregrina.棕尾别麻蝇凝集素的纯化及棕尾别麻蝇胚胎细胞系NIH-Sape-4培养基中麻蝇毒素的检测
Biochem J. 1987 Nov 15;248(1):217-22. doi: 10.1042/bj2480217.
3
Activation of latent ribonuclease in the fat-body of fleshfly (Sarcophaga peregrina) larvae on pupation.麻蝇（Sarcophaga peregrina）幼虫化蛹时脂肪体中潜在核糖核酸酶的激活。
Biochem J. 1981 Jun 15;196(3):699-703. doi: 10.1042/bj1960699.
4
Identification of 30-kDa fat body protein of Sarcophaga peregrina larvae selectively phosphorylated in the presence of 20-hydroxyecdysone as ribosomal protein S6.将嗜尸性麻蝇幼虫30 kDa脂肪体蛋白鉴定为在20-羟基蜕皮激素存在下被选择性磷酸化的核糖体蛋白S6。
J Biochem. 1986 Aug;100(2):493-8. doi: 10.1093/oxfordjournals.jbchem.a121738.
5
Differential expression of two abundant messenger RNAs during development of Sarcophaga peregrina.棕尾别麻蝇发育过程中两种丰富信使核糖核酸的差异表达
Dev Biol. 1983 Sep;99(1):145-51. doi: 10.1016/0012-1606(83)90261-0.
6
Mode of action of a bactericidal protein induced in the haemolymph of Sarcophaga peregrina (flesh-fly) larvae.在棕尾别麻蝇（肉蝇）幼虫血淋巴中诱导产生的一种杀菌蛋白的作用模式。
Biochem J. 1984 Aug 15;222(1):119-24. doi: 10.1042/bj2220119.
7
Humoral mediator-dependent activation of the Sarcophaga lectin gene.体液介质依赖性激活麻蝇凝集素基因。
FEBS Lett. 1988 May 9;232(1):163-6. doi: 10.1016/0014-5793(88)80409-5.
8
Purification and characterization of an antibacterial protein from haemolymph of Sarcophaga peregrina (flesh-fly) larvae.棕尾别麻蝇（肉蝇）幼虫血淋巴中一种抗菌蛋白的纯化与特性分析
Biochem J. 1983 Jun 1;211(3):727-34. doi: 10.1042/bj2110727.
9
Multi-gene structure of the storage protein genes of Sarcophaga peregrina.棕尾别麻蝇储存蛋白基因的多基因结构
J Mol Biol. 1984 Mar 25;174(1):19-29. doi: 10.1016/0022-2836(84)90362-0.
10
Identification of storage protein receptor and its precursor in the fat body membrane of Sarcophaga peregrina.
J Biol Chem. 1984 Oct 10;259(19):12107-11.

引用本文的文献

1
Stage- and tissue-specific expression of the genes encoding calliphorin, the major larval serum protein of Calliphora vicina.编码嗜尸丽蝇主要幼虫血清蛋白丽蝇蛋白的基因的阶段和组织特异性表达。
Rouxs Arch Dev Biol. 1986 Jul;195(5):290-295. doi: 10.1007/BF00376061.
2
Homologies of nucleotide sequences in the 5'-end regions of two developmentally regulated genes of Sarcophaga peregrina.棕尾别麻蝇两个发育调控基因5'-末端区域核苷酸序列的同源性
Nucleic Acids Res. 1986 Mar 25;14(6):2685-98. doi: 10.1093/nar/14.6.2685.
3
Purification of Sarcophaga (fleshfly) lectin and detection of sarcotoxins in the culture medium of NIH-Sape-4, an embryonic cell line of Sarcophaga peregrina.

本文引用的文献

1
Amplification of genes for chorion proteins during oogenesis in Drosophila melanogaster.黑腹果蝇卵子发生过程中绒毛膜蛋白基因的扩增
Proc Natl Acad Sci U S A. 1980 Feb;77(2):1096-100. doi: 10.1073/pnas.77.2.1096.
2
Developmental changes in the late larva of Calliphora stygia. IV. Uptake of plasma protein by the fat body.Stygia丽蝇晚期幼虫的发育变化。IV. 脂肪体对血浆蛋白的摄取。
Aust J Biol Sci. 1971 Apr;24(2):291-9. doi: 10.1071/bi9710291.
3
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
棕尾别麻蝇凝集素的纯化及棕尾别麻蝇胚胎细胞系NIH-Sape-4培养基中麻蝇毒素的检测
Biochem J. 1987 Nov 15;248(1):217-22. doi: 10.1042/bj2480217.
在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
4
Control of globin synthesis: the role of heme.珠蛋白合成的调控：血红素的作用。
J Mol Biol. 1972 May 28;66(3):471-81. doi: 10.1016/0022-2836(72)90427-5.
5
Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A.用葡萄球菌蛋白A-抗体吸附剂从细胞中快速分离抗原：抗体-抗原复合物与蛋白A相互作用的参数
J Immunol. 1975 Dec;115(6):1617-24.
6
An efficient mRNA-dependent translation system from reticulocyte lysates.一种来自网织红细胞裂解物的高效mRNA依赖性翻译系统。
Eur J Biochem. 1976 Aug 1;67(1):247-56. doi: 10.1111/j.1432-1033.1976.tb10656.x.
7
Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma.蛋白质跨膜转运。I. 小鼠骨髓瘤膜结合核糖体上经蛋白酶加工和未经加工的新生免疫球蛋白轻链的存在情况
J Cell Biol. 1975 Dec;67(3):835-51. doi: 10.1083/jcb.67.3.835.
8
Insect plasma proteins.昆虫血浆蛋白。
Annu Rev Biochem. 1978;47:779-817. doi: 10.1146/annurev.bi.47.070178.004023.
9
Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.在十二烷基硫酸钠中通过有限蛋白酶解进行肽图谱分析并通过凝胶电泳进行分析。
J Biol Chem. 1977 Feb 10;252(3):1102-6.