K-Herb Research Center, Korea Institute of Oriental Medicine, Yuseong-daero, Yuseong-gu, Daejeon, Republic of Korea.
J Sep Sci. 2017 May;40(9):2071-2079. doi: 10.1002/jssc.201601261. Epub 2017 Apr 18.
A rapid ultra-performance convergence chromatography method was developed for the quantitative determination of bioactive compounds in Aralia continentalis as quality control markers. Quantitative analysis indicated the presence of two major bioactive compounds: diterpenoid acids continentalic acid and kaurenoic acid. Using a Torus 1-aminoanthracene column, continentalic acid and kaurenoic acid were separated in less than 8 min. The method was validated with respect to precision, accuracy, and linearity according to the International Conference on Harmonization guidelines. The optimized method exhibited a good linear correlation (r > 0.996), excellent precision (RSD < 1.0%), and acceptable recoveries (99.97-100.26%). Limits of detection for continentalic acid and kaurenoic acid were 0.068 and 0.097 μg/mL, respectively, while their corresponding limits of quantitation were 0.207 and 0.295 μg/mL. The system performance of ultra-performance convergence chromatography was compared with that of conventional high-performance liquid chromatography with respect to analysis time and efficiency. The proposed method was found to be reliable and convenient for the quantitative analysis of continentalic acid and kaurenoic acid in A. continentalis from South Korea and A. pubescens from China. This study is expected to serve as a guideline for the quality control of Aralia continentalis.
建立了一种快速超高效并流色谱法，用于作为质量控制标志物对辽东楤木中的生物活性化合物进行定量测定。定量分析表明存在两种主要的生物活性化合物：二萜酸大陆酸和贝壳杉烯酸。使用 Torus 1-氨基蒽柱，在不到 8 分钟内即可分离出大陆酸和贝壳杉烯酸。该方法根据国际协调会议的指导原则进行了精密度、准确度和线性验证。优化后的方法表现出良好的线性相关性（r ＞ 0.996）、优异的精密度（RSD ＜ 1.0％）和可接受的回收率（99.97-100.26％）。大陆酸和贝壳杉烯酸的检测限分别为 0.068 和 0.097 μg/mL，定量限分别为 0.207 和 0.295 μg/mL。与传统高效液相色谱相比，超高效并流色谱的系统性能在分析时间和效率方面进行了比较。该方法被发现对于定量分析来自韩国的辽东楤木和中国的短梗五加中的大陆酸和贝壳杉烯酸是可靠且方便的。本研究有望为辽东楤木的质量控制提供指导。
