Research Institute for Sustainable Humanosphere, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan.
Cell Chem Biol. 2017 Mar 16;24(3):246-247. doi: 10.1016/j.chembiol.2017.03.007.
In this issue of Cell Chemical Biology, Lion et al. (2017) report a multiplexed labeling method to visualize plant cell wall lignification in vivo. This approach uses two different lignin precursor analogs tagged with azide and alkyne reporters that can be independently incorporated into cell walls, then differentially derivatized in vivo via two bioorthogonal click reactions: strain-promoted and copper-assisted azide-alkyne cycloadditions.
在本期《细胞化学生物学》中，Lion 等人（2017 年）报道了一种多重标记方法，可用于可视化体内植物细胞壁木质化。该方法使用两种带有叠氮化物和炔烃报告基团的不同木质素前体类似物，这些类似物可以独立地掺入细胞壁中，然后通过两种生物正交点击反应在体内进行差异衍生：应变促进和铜辅助的叠氮化物-炔烃环加成反应。
