Müggenburg Frederik, Müller Sabine
Institut für Biochemie, Universität Greifswald, Felix-Hausdorff-Straße 4, 17487, Greifswald, Germany.
Chem Rec. 2022 May;22(5):e202100322. doi: 10.1002/tcr.202100322. Epub 2022 Feb 21.
Azide-modified nucleosides are important building blocks for RNA and DNA functionalization by click chemistry based on azide-alkyne cycloaddition. This has put demand on synthetic chemistry to develop approaches for the preparation of azide-modified nucleoside derivatives. We review here the available methods for the synthesis of various nucleosides decorated with azido groups at the sugar residue or nucleobase, their incorporation into oligonucleotides and cellular RNAs, and their application in azide-alkyne cycloadditions for labelling and functionalization.
叠氮修饰的核苷是基于叠氮-炔环加成点击化学实现RNA和DNA功能化的重要构建模块。这对合成化学提出了需求，即开发制备叠氮修饰核苷衍生物的方法。我们在此综述了在糖残基或核苷碱基上带有叠氮基的各种核苷的合成方法、它们掺入寡核苷酸和细胞RNA中的情况，以及它们在用于标记和功能化的叠氮-炔环加成反应中的应用。
