Oberst R D, Stott J L, Blanchard-Channell M, Osburn B I
Department of Veterinary Pathology, University of California, Davis 95616.
Vet Microbiol. 1987 Oct;15(1-2):11-8. doi: 10.1016/0378-1135(87)90124-6.
Reassortants of bluetongue virus Serotype 11 (BTV-11) were isolated from a yearling heifer experimentally infected with two electrophoretically different strains (UC-2 and UC-8) by subcutaneous inoculation. Viruses were recovered by direct titration of sonicated blood samples onto Vero cell monolayers, which were overlaid with agarose and later plaque purified. The parental electropherotype of UC-8 was identified as the predominant virus strain during the infection; UC-2 was not isolated. UC-2 infectivity was shown by reassortants which contained genome segments that were identical in migration pattern to the parental UC-2 electropherotype. The observations demonstrate that segmental reassortment can occur during mixed infections in the bovine, between strains of the same BTV serotype.
从一头通过皮下接种实验感染了两种电泳不同毒株（UC - 2和UC - 8）的一岁小母牛中分离出了蓝舌病毒血清型11（BTV - 11）的重配体。通过将超声处理后的血样直接滴定到Vero细胞单层上回收病毒，Vero细胞单层用琼脂糖覆盖，随后进行蚀斑纯化。在感染期间，UC - 8的亲本电泳型被鉴定为主要病毒株；未分离到UC - 2。含有与亲本UC - 2电泳型迁移模式相同的基因组片段的重配体显示出UC - 2的感染性。这些观察结果表明，在牛的混合感染期间，同一BTV血清型的毒株之间可能会发生节段重配。
