Tweardy D J, Caracciolo D, Valtieri M, Rovera G
Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104.
Ann N Y Acad Sci. 1987;511:30-8. doi: 10.1111/j.1749-6632.1987.tb36235.x.
The conditioned media of 34 human tumor cell lines were screened for the ability to induce granulocyte-macrophage colonies in vitro in bone marrow cultures, to stimulate proliferation of a murine IL-3 dependent hemopoietic cell line (32D clone 3) and to stimulate thymidine incorporation in suspension cultures of acute myelogenous leukemia cells. Twelve tumor cell lines produced factors that were active in these assays. The conditioned medium of the glioblastoma cell line U87 MG was characterized in detail and found to contain G-CSF and GM-CSF. Cloning and sequencing of the U87 MG G-CSF indicated that it was derived from G-CSF b mRNA, which encodes a protein with a deletion of 3 amino acids at residues 36-38. The gene for G-CSF was mapped to human chromosome 17 band q21, a region involved in translocations frequently found in acute promyelocytic leukemia. G-CSF (U87MG) was able to induce granulocytic differentiation of the total population of a murine IL-3 dependent cell line, 32D clone 3; this effect was antagonized by IL-3. GM-CSF (U87-MG) supported the proliferation without inducing differentiation of two growth factor-dependent leukemic cell lines, TALL 101 and AML-193.
对34种人类肿瘤细胞系的条件培养基进行了筛选，以检测其在体外骨髓培养中诱导粒细胞-巨噬细胞集落的能力、刺激鼠IL-3依赖造血细胞系（32D克隆3）增殖的能力以及刺激急性髓性白血病细胞悬浮培养中胸苷掺入的能力。12种肿瘤细胞系产生了在这些检测中具有活性的因子。对胶质母细胞瘤细胞系U87 MG的条件培养基进行了详细表征，发现其含有G-CSF和GM-CSF。U87 MG G-CSF的克隆和测序表明它源自G-CSF b mRNA，该mRNA编码的蛋白质在第36-38位残基处缺失3个氨基酸。G-CSF基因定位于人类染色体17q21带，该区域在急性早幼粒细胞白血病中经常发生易位。G-CSF（U87MG）能够诱导鼠IL-3依赖细胞系32D克隆3的全部细胞群体发生粒细胞分化；这种作用被IL-3拮抗。GM-CSF（U87-MG）支持两种生长因子依赖的白血病细胞系TALL 101和AML-193的增殖而不诱导其分化。
