Koike K, Ogawa M, Ihle J N, Miyake T, Shimizu T, Miyajima A, Yokota T, Arai K
J Cell Physiol. 1987 Jun;131(3):458-64. doi: 10.1002/jcp.1041310319.
We studied the effects of murine recombinant granulocyte-macrophage colony-stimulating factor (GM-CSF) on murine hemopoiesis in methylcellulose culture. The GM-CSF was purified from cultures of Saccharomyces cerevisiae transfected with a cloned murine GM-CSF cDNA. In cultures of spleen cells from normal mice, only granulocyte-macrophage (GM) colonies were supported by GM-CSF. Blast cell colonies were the predominant type in cultures of spleen cells from 5-fluorouracil (5-FU)-treated mice. Dose-response studies revealed that maximal GM and blast cell colony formation is achieved with 100 U/ml GM-CSF. Blast cell colonies revealed variable but high replating efficiencies, and the secondary colonies included multilineage colonies. Serial replating of washed blast cell colonies in cultures with GM-CSF provided evidence for the direct effects of GM-CSF on the proliferation of multipotential blast cells. A combination of GM-CSF and interleukin-3 (IL-3) did not increase the number of blast cell colonies over the level supported by IL-3. This observation indicates that the progenitors for blast cell colonies that responded to GM-CSF are a subpopulation of multipotential progenitors that are supported by IL-3. Cytological studies of colonies derived from GM-CSF and/or IL-3 suggest that the eosinophilopoietic ability of murine GM-CSF is less than that of IL-3.
我们在甲基纤维素培养体系中研究了小鼠重组粒细胞-巨噬细胞集落刺激因子（GM-CSF）对小鼠造血的影响。GM-CSF是从转染了克隆的小鼠GM-CSF cDNA的酿酒酵母培养物中纯化得到的。在正常小鼠脾细胞培养物中，GM-CSF仅支持粒细胞-巨噬细胞（GM）集落的形成。在经5-氟尿嘧啶（5-FU）处理的小鼠脾细胞培养物中，原始细胞集落是主要类型。剂量反应研究表明，100 U/ml的GM-CSF可实现最大的GM和原始细胞集落形成。原始细胞集落显示出可变但较高的再接种效率，并且次级集落包括多谱系集落。在用GM-CSF培养的体系中对洗涤后的原始细胞集落进行连续再接种，为GM-CSF对多能原始细胞增殖的直接作用提供了证据。GM-CSF和白细胞介素-3（IL-3）的组合并未使原始细胞集落的数量超过IL-3所支持的水平。这一观察结果表明，对GM-CSF有反应的原始细胞集落的祖细胞是由IL-3支持的多能祖细胞的一个亚群。对源自GM-CSF和/或IL-3的集落进行的细胞学研究表明，小鼠GM-CSF的嗜酸性粒细胞生成能力低于IL-3。
