Turk S R, Cook P D, Reinke C M, Drach J C
Department of Oral Biology, School of Dentistry, University of Michigan, Ann Arbor 48109.
Antiviral Res. 1987 Sep;8(2):97-102. doi: 10.1016/0166-3542(87)90080-5.
Preliminary studies of the biochemical basis for the antiviral activity of the pyrrolo[2,3-d]pyrimidine nucleoside ara-tubercidin were conducted. Herpes simplex virus DNA synthesis was 3-fold more sensitive to inhibition by ara-tubercidin than was cellular DNA synthesis. Partially purified herpes DNA polymerases were more sensitive to inhibition by ara-tubercidin 5'-triphosphate than were cellular polymerases alpha and beta. Inhibition of viral DNA polymerase was competitive with dATP and noncompetitive with dTTP. The results suggest that the viral DNA polymerase plays a significant role in the antiviral activity of ara-tubercidin.
对吡咯并[2,3-d]嘧啶核苷阿糖结核菌素抗病毒活性的生化基础进行了初步研究。单纯疱疹病毒DNA合成对阿糖结核菌素抑制作用的敏感性比对细胞DNA合成的敏感性高3倍。部分纯化的疱疹DNA聚合酶对阿糖结核菌素5'-三磷酸的抑制作用比细胞聚合酶α和β更敏感。病毒DNA聚合酶的抑制作用与dATP呈竞争性，与dTTP呈非竞争性。结果表明，病毒DNA聚合酶在阿糖结核菌素的抗病毒活性中起重要作用。
