Furman P A, St Clair M H, Fyfe J A, Rideout J L, Keller P M, Elion G B
J Virol. 1979 Oct;32(1):72-7. doi: 10.1128/JVI.32.1.72-77.1979.
The effect of the nucleoside analog 9-(2-hydroxyethoxymethyl)guanine (acycloguanosine) on herpes simplex virus type 1 DNA synthesis was examined. Acycloguanosine inhibited herpesvirus DNA synthesis in virus-infected cells. The synthesis of host cell DNA was only partially inhibited in actively growing cells at acycloguanosine concentrations several hundred-fold greater than the 50% effective dose for herpes simplex virus type 1. Studies using partially purified enzymes revealed that the triphosphate of this compound inhibited the virus-induced DNA polymerases (DNA nucleotidyltransferases) to a greater degree than the DNA polymerase of the host cell, that the inhibition was dependent upon the base composition of the template, and that the triphosphate was a better substrate for the virus-induced polymerases than for the alpha cellular DNA polymerases.
研究了核苷类似物9-(2-羟乙氧甲基)鸟嘌呤（阿昔洛韦）对1型单纯疱疹病毒DNA合成的影响。阿昔洛韦可抑制病毒感染细胞中的疱疹病毒DNA合成。在阿昔洛韦浓度比1型单纯疱疹病毒的50%有效剂量高数百倍时，活跃生长的细胞中宿主细胞DNA的合成仅受到部分抑制。使用部分纯化酶进行的研究表明，该化合物的三磷酸盐对病毒诱导的DNA聚合酶（DNA核苷酸转移酶）的抑制作用比对宿主细胞的DNA聚合酶更强，这种抑制作用取决于模板的碱基组成，并且该三磷酸盐是病毒诱导聚合酶比α细胞DNA聚合酶更好的底物。
