9-(2-羟乙氧甲基)鸟嘌呤及其三磷酸酯对单纯疱疹病毒诱导的DNA聚合酶活性和病毒DNA复制的抑制作用。
Inhibition of herpes simplex virus-induced DNA polymerase activity and viral DNA replication by 9-(2-hydroxyethoxymethyl)guanine and its triphosphate.
作者信息
Furman P A, St Clair M H, Fyfe J A, Rideout J L, Keller P M, Elion G B
出版信息
J Virol. 1979 Oct;32(1):72-7. doi: 10.1128/JVI.32.1.72-77.1979.
Abstract
The effect of the nucleoside analog 9-(2-hydroxyethoxymethyl)guanine (acycloguanosine) on herpes simplex virus type 1 DNA synthesis was examined. Acycloguanosine inhibited herpesvirus DNA synthesis in virus-infected cells. The synthesis of host cell DNA was only partially inhibited in actively growing cells at acycloguanosine concentrations several hundred-fold greater than the 50% effective dose for herpes simplex virus type 1. Studies using partially purified enzymes revealed that the triphosphate of this compound inhibited the virus-induced DNA polymerases (DNA nucleotidyltransferases) to a greater degree than the DNA polymerase of the host cell, that the inhibition was dependent upon the base composition of the template, and that the triphosphate was a better substrate for the virus-induced polymerases than for the alpha cellular DNA polymerases.
摘要
研究了核苷类似物9-(2-羟乙氧甲基)鸟嘌呤（阿昔洛韦）对1型单纯疱疹病毒DNA合成的影响。阿昔洛韦可抑制病毒感染细胞中的疱疹病毒DNA合成。在阿昔洛韦浓度比1型单纯疱疹病毒的50%有效剂量高数百倍时，活跃生长的细胞中宿主细胞DNA的合成仅受到部分抑制。使用部分纯化酶进行的研究表明，该化合物的三磷酸盐对病毒诱导的DNA聚合酶（DNA核苷酸转移酶）的抑制作用比对宿主细胞的DNA聚合酶更强，这种抑制作用取决于模板的碱基组成，并且该三磷酸盐是病毒诱导聚合酶比α细胞DNA聚合酶更好的底物。
相似文献
1
Inhibition of herpes simplex virus-induced DNA polymerase activity and viral DNA replication by 9-(2-hydroxyethoxymethyl)guanine and its triphosphate.9-(2-羟乙氧甲基)鸟嘌呤及其三磷酸酯对单纯疱疹病毒诱导的DNA聚合酶活性和病毒DNA复制的抑制作用。
J Virol. 1979 Oct;32(1):72-7. doi: 10.1128/JVI.32.1.72-77.1979.
2
3
Selectivity of action of an antiherpetic agent, 9-(2-hydroxyethoxymethyl) guanine.一种抗疱疹剂9-(2-羟乙氧甲基)鸟嘌呤的作用选择性
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5716-20. doi: 10.1073/pnas.74.12.5716.
4
Involvement of eucaryotic deoxyribonucleic acid polymerases alpha and gamma in the replication of cellular and viral deoxyribonucleic acid.真核生物脱氧核糖核酸聚合酶α和γ参与细胞和病毒脱氧核糖核酸的复制。
Biochemistry. 1979 Oct 2;18(20):4431-43. doi: 10.1021/bi00587a025.
5
6
Mode of antiviral action of penciclovir in MRC-5 cells infected with herpes simplex virus type 1 (HSV-1), HSV-2, and varicella-zoster virus.喷昔洛韦对感染1型单纯疱疹病毒（HSV-1）、2型单纯疱疹病毒（HSV-2）和水痘-带状疱疹病毒的MRC-5细胞的抗病毒作用模式
Antimicrob Agents Chemother. 1992 Dec;36(12):2747-57. doi: 10.1128/AAC.36.12.2747.
7
Inhibition of herpes simplex virus DNA replication by ara-tubercidin.
Antiviral Res. 1987 Sep;8(2):97-102. doi: 10.1016/0166-3542(87)90080-5.
8
Inhibition of cellular alpha DNA polymerase and herpes simplex virus-induced DNA polymerases by the triphosphate of BW759U.BW759U的三磷酸盐对细胞α-DNA聚合酶和单纯疱疹病毒诱导的DNA聚合酶的抑制作用。
Antimicrob Agents Chemother. 1984 Feb;25(2):191-4. doi: 10.1128/AAC.25.2.191.
9
On the complex nature of the antiviral activity of coumermycin A1: its interference with the replication of herpes simplex virus type 1.论香豆霉素A1抗病毒活性的复杂性质：其对单纯疱疹病毒1型复制的干扰
Antiviral Res. 1986 Jan;6(1):19-32. doi: 10.1016/0166-3542(86)90036-7.
10
Metabolism and mode of action of (R)-9-(3,4-dihydroxybutyl)guanine in herpes simplex virus-infected vero cells.（R）-9-（3,4-二羟基丁基）鸟嘌呤在单纯疱疹病毒感染的非洲绿猴肾细胞中的代谢及作用方式
J Biol Chem. 1986 Feb 15;261(5):2134-9.
引用本文的文献
1
Valorization of a Natural Compound Library in Exploring Potential Marburg Virus VP35 Cofactor Inhibitors via an In Silico Drug Discovery Strategy.通过计算机辅助药物发现策略对天然化合物库进行价值评估以探索潜在的马尔堡病毒VP35辅助因子抑制剂
Curr Issues Mol Biol. 2025 Jul 2;47(7):506. doi: 10.3390/cimb47070506.
2
Chapter 3 Antiviral drugs: general considerations.第3章 抗病毒药物：一般考虑因素。
Perspect Med Virol. 1985;1:93-126. doi: 10.1016/S0168-7069(08)70011-9. Epub 2008 May 29.
3
Anti-CyHV-3 Effect of Fluorescent, Tricyclic Derivative of Acyclovir 6-(4-MeOPh)-TACV .阿昔洛韦6-(4-甲氧基苯基)-三环衍生物（6-(4-MeOPh)-TACV）的荧光特性及其抗鲤疱疹病毒3型的作用
J Vet Res. 2019 Oct 24;63(4):513-518. doi: 10.2478/jvetres-2019-0065. eCollection 2019 Dec.
4
RNA Polymerase II Promoter-Proximal Pausing and Release to Elongation Are Key Steps Regulating Herpes Simplex Virus 1 Transcription.RNA 聚合酶 II 启动子近端暂停和释放到延伸是调节单纯疱疹病毒 1 转录的关键步骤。
J Virol. 2020 Feb 14;94(5). doi: 10.1128/JVI.02035-19.
5
Retinitis associated with double infection of Epstein-Barr virus and varicella-zoster virus: A case report.与爱泼斯坦-巴尔病毒和水痘-带状疱疹病毒双重感染相关的视网膜炎：一例报告。
Medicine (Baltimore). 2018 Aug;97(31):e11663. doi: 10.1097/MD.0000000000011663.
6
Rad51 Degradation: Role in Oncolytic Virus-Poly(ADP-Ribose) Polymerase Inhibitor Combination Therapy in Glioblastoma.Rad51降解：在胶质母细胞瘤溶瘤病毒-聚（ADP-核糖）聚合酶抑制剂联合治疗中的作用
J Natl Cancer Inst. 2017 Mar 1;109(3):1-13. doi: 10.1093/jnci/djw229.
7
8
Turning an antiviral into an anticancer drug: nanoparticle delivery of acyclovir monophosphate.将抗病毒药物转化为抗癌药物：单磷酸阿昔洛韦的纳米颗粒递药系统。
J Control Release. 2013 Sep 28;170(3):414-20. doi: 10.1016/j.jconrel.2013.06.009. Epub 2013 Jun 18.
9
Direct evidence that HSV DNA damaged by ultraviolet (UV) irradiation can be repaired in a cell type-dependent manner.直接证据表明，紫外线（UV）照射损伤的单纯疱疹病毒（HSV）DNA 可以在依赖于细胞类型的方式下修复。
J Neurovirol. 2012 Jun;18(3):231-43. doi: 10.1007/s13365-012-0105-2. Epub 2012 May 12.
10
Topical Acyclovir for Recurrent Herpes Labialis in Primary Care: Critical appraisal.局部用阿昔洛韦治疗原发性疱疹性唇炎的复发：批判性评价。
Can Fam Physician. 1991 Jan;37:92-8.
本文引用的文献
1
A novel method for phosphorylation of nucleosides to 5'-nucleotides.一种将核苷磷酸化为5'-核苷酸的新方法。
Tetrahedron Lett. 1967 Dec;50:5065-8. doi: 10.1016/s0040-4039(01)89915-9.
2
Kinetics and intermediates in the intracellular synthesis of bacteriophage T4 deoxyribonucleic acid.噬菌体T4脱氧核糖核酸细胞内合成的动力学及中间产物
J Mol Biol. 1970 Jun 14;50(2):235-61. doi: 10.1016/0022-2836(70)90190-7.
3
Pharmacology of viruses.病毒药理学。
Annu Rev Pharmacol. 1970;10:143-70. doi: 10.1146/annurev.pa.10.040170.001043.
4
In vitro transcription of Escherichia coli ribosomal RNA genes.大肠杆菌核糖体RNA基因的体外转录
Nature. 1972 Feb 11;235(5337):329-33. doi: 10.1038/235329a0.
5
Characterization of herpes simplex virus-induced deoxyribonucleic acid polymerase.单纯疱疹病毒诱导的脱氧核糖核酸聚合酶的特性分析
J Biol Chem. 1973 Sep 25;248(18):6270-7.
6
Size, composition, and structure of the deoxyribonucleic acid of herpes simplex virus subtypes 1 and 2.单纯疱疹病毒1型和2型脱氧核糖核酸的大小、组成及结构
J Virol. 1971 Aug;8(2):125-32. doi: 10.1128/JVI.8.2.125-132.1971.
7
Detection of Epstein-Barr viral genome in nonproductive cells.在非增殖性细胞中检测爱泼斯坦-巴尔病毒基因组。
Nat New Biol. 1971 Sep 22;233(38):103-6. doi: 10.1038/newbio233103a0.
8
Enzymatic synthesis of deoxyribonucleic acid. XXXIV. Termination of chain growth by a 2',3'-dideoxyribonucleotide.脱氧核糖核酸的酶促合成。三十四。2',3'-二脱氧核糖核苷酸对链生长的终止作用。
Biochemistry. 1969 Dec;8(12):4897-904. doi: 10.1021/bi00840a037.
9
Human cytomegalovirus. I. Purification and characterization of viral DNA.人巨细胞病毒。一、病毒DNA的纯化与特性分析。
J Virol. 1973 Dec;12(6):1473-81. doi: 10.1128/JVI.12.6.1473-1481.1973.
10
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.一种利用蛋白质 - 染料结合原理对微克级蛋白质进行定量的快速灵敏方法。
Anal Biochem. 1976 May 7;72:248-54. doi: 10.1016/0003-2697(76)90527-3.
Zotero 插件