Piasecki E
Laboratory of Tumor Virology, Polish Academy of Sciences, Wroclaw.
Arch Immunol Ther Exp (Warsz). 1987;35(4):473-88.
The effects of the mixtures of human, porcine and murine interferons (IFNs) alpha, beta and gamma and bovine IFN-alpha were studied in human, porcine and murine cells. The synergistic action of the mixtures of IFN-alpha/beta and IFN-gamma occurred not only in the homologous but also in the heterologous systems. The synergistic effect was observed when one of the IFNs was of alpha/beta type and the other one of gamma type and when both of them were active in the target cells, regardless of their species origin. Because PoIFN-alpha is active in the cells of numerous mammalian species, it is a good tool for the studies on the synergistic effects. The synergistic action of the mixture of IFNs was abolished after inactivation of one of the IFNs. Specific inactivation of IFN-alpha/beta in the mixture was accomplished by incubation with anti-IFN sera and in the case of IFN-gamma, by acidifying to pH 2.
研究了人、猪和鼠的α、β和γ干扰素（IFN）混合物以及牛IFN-α在人、猪和鼠细胞中的作用。IFN-α/β与IFN-γ混合物的协同作用不仅发生在同源系统中，也发生在异源系统中。当其中一种IFN为α/β型而另一种为γ型，且两者在靶细胞中均有活性时，无论其物种来源如何，均观察到协同效应。由于猪IFN-α在众多哺乳动物物种的细胞中具有活性，因此它是研究协同效应的良好工具。其中一种IFN失活后，IFN混合物的协同作用消失。通过与抗IFN血清孵育可实现混合物中IFN-α/β的特异性失活，而对于IFN-γ，通过酸化至pH 2来实现。
