Xiao C Z, Chen Z L, Wu B Z
Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing, China.
Arch Immunol Ther Exp (Warsz). 1989;37(5-6):513-8.
It was found that development of antiviral state in cells activated by IFN alpha and beta were quite rapid. After 6-7 h treatment IFN alpha and IFN beta protected cells completely and their effects lasted for another 9-12 h after removing them from the cultures. Then the effect declined, but some protective action remained after 48 h of incubation. IFN-gamma was different from IFN alpha or beta. It activated cells much more slowly, and could not protect cells completely before 24 h of treatment. When we compared three concentrations of IFN-alpha, the cells could not be protected completely by 5 IU/ml, and kinetics were similar with 250 and 25 IU/ml. When we added anti-IFN-alpha serum before IFN treatment, the development of the antiviral state was inhibited. The results suggested that one should adopt different ways when titrating and using different forms of IFN, and it might not be needed to use maximal tolerated doses and daily administrations, and to keep the high level of IFN for a long time. We think that these results might be useful for the clinicians considering the optimal schedule for IFN treatment.
研究发现，由α干扰素和β干扰素激活的细胞中抗病毒状态的发展相当迅速。经过6 - 7小时的处理，α干扰素和β干扰素能完全保护细胞，并且在将它们从培养物中去除后，其作用还能持续另外9 - 12小时。然后作用下降，但在孵育48小时后仍有一些保护作用。γ干扰素与α干扰素或β干扰素不同。它激活细胞的速度要慢得多，并且在处理24小时之前不能完全保护细胞。当我们比较三种浓度的α干扰素时，5 IU/ml不能完全保护细胞，250 IU/ml和25 IU/ml的动力学相似。当我们在干扰素处理前加入抗α干扰素血清时，抗病毒状态的发展受到抑制。结果表明，在滴定和使用不同形式的干扰素时应采用不同的方法，可能不需要使用最大耐受剂量和每日给药，也不需要长时间维持高浓度的干扰素。我们认为这些结果可能对临床医生考虑干扰素治疗的最佳方案有用。
