Bánhegyi G, Garzó T, Mészáros G, Faragó A, Antoni F, Mandl J
1st Institute of Biochemistry, Semmelweis University, Medical School, Budapest, Hungary.
Biochem Pharmacol. 1988 Mar 1;37(5):849-54. doi: 10.1016/0006-2952(88)90171-2.
The possibility of a short-term cAMP-dependent regulation of mixed-function oxidation and of glucuronide formation was investigated in isolated mouse hepatocytes and in mouse liver microsomal membranes. N6, O2-dibutyryl cAMP (in accordance with its increasing effect on gluconeogenesis) decreased aminopyrine oxidation and p-nitrophenol conjugation in isolated hepatocytes, while the phenolphthalein conjugation remained unaltered. Similar to dibutyryl cAMP the Ca2+ ionophore A 23187 also decreased aminopyrine oxidation. In cell-free systems the phosphorylation of isolated microsomal membranes by the exogenous cAMP-dependent protein kinase was inhibitory on aminopyrine oxidation and p-nitrophenol glucuronide formation but aniline oxidation and phenolphthalein glucuronidation were not affected. The correlation between the negative cAMP-dependent control of certain processes of biotransformation and the positive cAMP-dependent regulation of gluconeogenesis is discussed.
在分离的小鼠肝细胞和小鼠肝微粒体膜中，研究了混合功能氧化和葡糖醛酸形成的短期cAMP依赖性调节的可能性。N6，O2-二丁酰cAMP（与其对糖异生的增强作用一致）降低了分离肝细胞中氨基比林的氧化和对硝基苯酚的结合，而酚酞的结合保持不变。与二丁酰cAMP相似，Ca2+离子载体A 23187也降低了氨基比林的氧化。在无细胞系统中，外源性cAMP依赖性蛋白激酶对分离的微粒体膜的磷酸化抑制了氨基比林的氧化和对硝基苯酚葡糖醛酸的形成，但苯胺氧化和酚酞葡糖醛酸化不受影响。讨论了生物转化某些过程的负cAMP依赖性控制与糖异生的正cAMP依赖性调节之间的相关性。
