螺旋体属在其病毒、质粒和基因组之间共享共同的DNA序列。

Spiroplasma species share common DNA sequences among their viruses, plasmids and genomes.

作者信息

Ranhand J M, Nur I, Rose D L, Tully J G

机构信息

Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, Frederick, MD 21701.

出版信息

Ann Inst Pasteur Microbiol. 1987 Sep-Oct;138(5):509-22. doi: 10.1016/0769-2609(87)90037-8.

DOI:10.1016/0769-2609(87)90037-8

PMID:2830894

Abstract

Alkaline-Southern-blot analyses showed that a spiroplasma plasmid, pRA1, obtained from Spiroplasma citri (Maroc-R8A2), contained DNA sequences that were homologous to spiroplasma type 3 viruses (SV3) obtained from S. citri (Maroc-R8A2), S. citri (608) and S. mirum (SMCA). In addition, pRA1 and SV3(608) DNA shared common, but not necessarily related, sequences with extrachromosomal DNA derived from 11 Spiroplasma species or strains. Furthermore, SV3(608) had DNA homology with the chromosome from 6 distinct spiroplasmas but not with chromosomal DNA from eight other Spiroplasma species or strains. The biological function of these common sequences is unknown.

摘要

碱性 Southern 印迹分析表明，从柑橘螺原体（摩洛哥-R8A2）获得的一种螺原体质粒 pRA1，含有与从柑橘螺原体（摩洛哥-R8A2）、柑橘螺原体（608）和微小螺原体（SMCA）获得的 3 型螺原体病毒（SV3）同源的 DNA 序列。此外，pRA1 和 SV3(608) DNA 与来自 11 种螺原体物种或菌株的染色体外 DNA 共享共同但不一定相关的序列。此外，SV3(608) 与 6 种不同螺原体的染色体具有 DNA 同源性，但与其他 8 种螺原体物种或菌株的染色体 DNA 没有同源性。这些共同序列的生物学功能尚不清楚。