Heil G, Ganser A, Raghavachar A, Kurrle E, Heit W, Hoelzer D, Heimpel H
Department of Internal Medicine III, University of Ulm, F.R.G.
Br J Haematol. 1988 Jan;68(1):23-32. doi: 10.1111/j.1365-2141.1988.tb04174.x.
For further analysis of their lineage affinity the differentiation capacity of five cases of acute unclassified leukaemias were studied in vitro using 5 nM 12-O-tetradecanoyl-phorbol-13-acetate as an inducing agent. After 4 d in vitro a co-expression of both myeloid and early B-lymphoid or myeloid and early T-lymphoid antigens on the same population of cells was found. While ultrastructural analysis of the blasts prior to culture revealed the morphology of undifferentiated blasts without any specific endogenous peroxidase activity, the cultured blasts displayed monoblastic features and myeloperoxidase activity after 4 d in vitro. Since this type of peroxidase is highly specific for the myelomonocytic lineage the data indicate myelomonocytoid differentiation of all five cases studied. The co-expression of lymphoid as well as myeloid antigens and myeloperoxidase by the same blasts after culture could be indicative of bilineage differentiation capacity characterizing the original blasts as bipotential progenitor cells.
为了进一步分析其谱系亲和力，使用5 nM 12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯作为诱导剂，在体外研究了5例急性未分类白血病的分化能力。体外培养4天后，在同一细胞群体上发现了髓系和早期B淋巴细胞或髓系和早期T淋巴细胞抗原的共表达。虽然培养前原始细胞的超微结构分析显示未分化原始细胞的形态，且无任何特异性内源性过氧化物酶活性，但体外培养4天后，培养的原始细胞呈现单核细胞特征并具有髓过氧化物酶活性。由于这种类型的过氧化物酶对髓单核细胞谱系具有高度特异性，数据表明所研究的所有5例病例均有髓单核细胞样分化。培养后同一原始细胞同时表达淋巴样和髓系抗原以及髓过氧化物酶，可能表明具有双谱系分化能力，将原始细胞表征为双能祖细胞。
