Verkleij J A C, de Boer A M, Lugtenborg T F
Biologisch Laboratorium, Vrije Universiteit, Postbus 7161, 1007 MC, Amsterdam, The Netherlands.
Oecologia. 1980 Sep;46(3):354-359. doi: 10.1007/BF00346264.
Isoenzyme analyses were carried out on the hypotetraploid Stellaria media and the diploid Stellaria pallida occurring on abandoned sandy fields in the coastal dunes of the Netherlands. Amylase isoenzymes in germinating seeds of Stellaria media showed a striking intrapopulational variability, which is in accordance with the germination behaviour of the different subpopulations. In contrast to these findings no intrapopulational variation nor developmental change in isoenzyme pattern could be detected on basis of 14 other analysed leaf enzymes. For five enzymes there was a difference in isoenzyme pattern between the species Stellaria media and Stellaria pallida. Of these five enzymes two showed an interpopulational variation in Stellaria media. The fact that there was almost no variability of the isoenzyme pattern in Stellaria pallida could be explained by the permanent autogamous (cleistogamous) state of the species. Effects of polyploidy on the isoenzyme pattern and its activity could not be demonstrated. Consequences of the extreme difference between the monomorphic pattern of the leaf enzymes as opposed to the polymorphism of the amylase are discussed in view of the difference in germination ecology and lifecycle strategy of Stellaria media subpopulations.
对荷兰海岸沙丘废弃沙地上生长的亚四倍体繁缕和二倍体苍白繁缕进行了同工酶分析。繁缕萌发种子中的淀粉酶同工酶表现出显著的种群内变异性，这与不同亚种群的萌发行为一致。与这些发现相反，基于其他14种分析的叶片酶，未检测到种群内同工酶模式的变化或发育变化。在繁缕和苍白繁缕这两个物种之间，有五种酶的同工酶模式存在差异。在这五种酶中，有两种在繁缕中表现出种群间变异。苍白繁缕同工酶模式几乎没有变异性这一事实，可以用该物种永久自花授粉（闭花受精）的状态来解释。未证明多倍体对同工酶模式及其活性的影响。鉴于繁缕亚种群在萌发生态学和生命周期策略上的差异，讨论了叶片酶单态模式与淀粉酶多态性之间极端差异的后果。
