Schulz T F, Mitterer M, Vogetseder W, Böck G, Myones B L, Dierich M P
Institute for Hygiene, Innsbruck, Austria.
Eur J Immunol. 1988 Jan;18(1):7-11. doi: 10.1002/eji.1830180103.
A monoclonal antibody, 7F7, raised against the Raji B lymphoblastoid cell line reacted with about 35% of peripheral blood B cells, germinal center B cells, follicular dendritic cells and some vascular endothelial cells. Although not found on resting T cells this antigen was strongly expressed on CD4+ and CD8+ T cells activated with phytohemagglutinin, pokeweed mitogen and anti-T3, and its expression on B cells was enhanced after stimulation with pokeweed mitogen. It is strongly expressed 24 h after stimulation with phytohemagglutinin and its expression is reduced but not eliminated by cyclosporin A treatment. The molecule defined by antibody 7F7 is found on some, but not all, B cell lines, on an HTLV-I-transformed T cell line and on the promyelocytic cell line U937. Immunoprecipitation from externally and metabolically labeled Raji cells revealed a single-chain molecule of 85 kDa.
一种针对拉吉B淋巴母细胞系产生的单克隆抗体7F7，可与约35%的外周血B细胞、生发中心B细胞、滤泡树突状细胞及一些血管内皮细胞发生反应。虽然在静止T细胞上未发现该抗原，但在用植物血凝素、商陆有丝分裂原和抗T3激活的CD4⁺和CD8⁺T细胞上强烈表达，并且在用商陆有丝分裂原刺激后其在B细胞上的表达增强。在用植物血凝素刺激24小时后强烈表达，并且其表达在用环孢素A处理后降低但未消除。抗体7F7所定义的分子在一些（但不是全部）B细胞系、一种人嗜T细胞病毒I型转化的T细胞系及早幼粒细胞系U937上发现。从经外部和代谢标记的拉吉细胞进行免疫沉淀显示出一个85 kDa的单链分子。
