Lister A, Usher M B, Block W
British Antarctic Survey, Natural Environment Research Council, High Cross, Madingley Road, CB3 OET, Cambridge, UK.
Department of Biology, University of York, YO1 5DD, York, UK.
Oecologia. 1987 May;72(2):185-191. doi: 10.1007/BF00379265.
Polyacrylamide gel electrophoresis and subsequent esterase staining provide an alternative to immunological methods for investigating the diet of microarthropods which cannot be observed directly. Attack rates in field populations of Antarctic microarthropods by the predator Gamasellus racovitzai were calculated by proportional and quantitative analyses of electrophoresis results. Results were quantified by transmission densitometry and a digestion curve for ingested prey esterases was defined. An exponential decay model of digestion, incorporating meal size, was used, and a method potentially enabling both the time since feeding and the composite meal size to be estimated for individual predators is described. These methods are applied to samples of predatory mites collected from the Antarctic Peninsula. Although estimated attack rates were low, about 0.1-0.3 prey per predator per day, they may be sufficient to cause severe local reduction in prey density.
聚丙烯酰胺凝胶电泳及随后的酯酶染色为研究无法直接观察的微型节肢动物的食性提供了一种替代免疫方法。通过对电泳结果进行比例和定量分析，计算了捕食者拉氏革螨对南极微型节肢动物野外种群的攻击率。通过透射光密度测定法对结果进行定量，并确定了摄入猎物酯酶的消化曲线。使用了一个包含食量的指数衰减消化模型，并描述了一种有可能为个体捕食者估计进食后时间和综合食量的方法。这些方法应用于从南极半岛采集的捕食性螨类样本。尽管估计的攻击率较低，约为每个捕食者每天0.1 - 0.3个猎物，但它们可能足以导致猎物密度在局部严重下降。
