Belcourt A
J Dent Res. 1979 Mar;58(Spec Issue B):986-7. doi: 10.1177/00220345790580025301.
Pure enamel was prepared using an original microdissection technic. Protein concentration was 375 microgram per gram of enamel. Polyacrylamide gel electrophoresis showed a single fast-migrating zone containing a thin double band. Ultracentrifugation studies suggested that the proteins were of low molecular weight or of weak density. Absorption spectra showed a strong absorbance at 260nm. Amino acid analyses yielded a composition of 25% Gly, 13.5% Glu, 11% Ser, 11% Pro, 2% Cys and 2% Hyp. A glucidic content of 15% was estimated and glucose, galactose, mannose and fucose were identified. The organic matrix of enamel seemed to be constituted of two major glycoproteins probably fibrous but different from keratin.
采用一种原始的显微切割技术制备了纯牙釉质。蛋白质浓度为每克牙釉质375微克。聚丙烯酰胺凝胶电泳显示出一个单一的快速迁移区，其中包含一条细的双链带。超速离心研究表明，这些蛋白质分子量低或密度小。吸收光谱显示在260nm处有强吸收。氨基酸分析得出的组成是：25%的甘氨酸、13.5%的谷氨酸、11%的丝氨酸、11%的脯氨酸、2%的半胱氨酸和2%的羟脯氨酸。估计含糖量为15%，并鉴定出葡萄糖、半乳糖、甘露糖和岩藻糖。牙釉质的有机基质似乎由两种主要的糖蛋白组成，可能是纤维状的，但与角蛋白不同。
