Kato-Noguchi Hisashi, Nakamura Keisuke, Ohno Osamu, Suenaga Kiyotake, Okuda Nobuyuki
Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Miki, Kagawa 761-0795, Japan.
Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Miki, Kagawa 761-0795, Japan.
J Plant Physiol. 2017 Jun;213:23-29. doi: 10.1016/j.jplph.2017.02.011. Epub 2017 Mar 1.
Asparagus (Asparagus officinalis L.) is a perennial vegetable, but its crop productivity and quality decrease gradually. One possible reason for "asparagus decline" is thought to be the autotoxicity of asparagus. However, the autotoxic property of asparagus rhizomes remains unknown. The objective of this study was to determine the potential role of rhizomes in the autotoxicity of asparagus. An aqueous methanol extract of asparagus rhizomes inhibited the growth of asparagus seedlings and six other test plants in a concentration-dependent manners: garden cress (Lepidum sativum L.), lettuce (Lactuca sativa L.), alfalfa (Medicago sativa L.), ryegrass (Lolium multiflorum Lam.), timothy (Phleum pratense L.) and barnyardgrass (Echinochloa crus-galli (L.) Beauv.). These results suggest that asparagus rhizomes contain autotoxic compounds. The extract was purified through several chromatographic steps with monitoring the autotoxic activity, and p-coumaric acid and iso-agatharesinol were isolated. These compounds inhibited the shoot and root growth of asparagus and two other test plants, garden cress and ryegrass, at concentrations higher than 0.1mM. The concentrations required for 50% inhibition of the root and shoot growth of these test plants ranged from 0.36 to 0.85mM and 0.41-1.22mM for p-coumaric acid and iso-agatharesinol, respectively. Therefore, these compounds may contribute to the autotoxicity caused by asparagus rhizomes and may be involved in "asparagus decline".
芦笋（Asparagus officinalis L.）是一种多年生蔬菜，但其作物产量和品质会逐渐下降。“芦笋衰退”的一个可能原因被认为是芦笋的自毒作用。然而，芦笋根茎的自毒特性尚不清楚。本研究的目的是确定根茎在芦笋自毒作用中的潜在作用。芦笋根茎的甲醇水提取物以浓度依赖的方式抑制芦笋幼苗和其他六种受试植物的生长：水芹（Lepidum sativum L.）、生菜（Lactuca sativa L.）、苜蓿（Medicago sativa L.）、黑麦草（Lolium multiflorum Lam.）、梯牧草（Phleum pratense L.）和稗草（Echinochloa crus-galli (L.) Beauv.）。这些结果表明芦笋根茎含有自毒化合物。通过几个色谱步骤对提取物进行纯化并监测自毒活性，分离出了对香豆酸和异落叶松脂醇。这些化合物在浓度高于0.1mM时抑制芦笋以及另外两种受试植物水芹和黑麦草的地上部和根部生长。对香豆酸和异落叶松脂醇分别使这些受试植物根部和地上部生长受到50%抑制所需的浓度范围为0.36至0.85mM和0.41 - 1.22mM。因此这些化合物可能导致芦笋根茎引起的自毒作用并可能与“芦笋衰退”有关。
