Hara Keigo, Miyazaki Tatsuya, Yokobori Takehiko, Yoshida Tomonori, Kumakura Yuji, Honjyo Hiroaki, Sakai Makoto, Sohda Makoto, Fukuchi Minoru, Kuwano Hiroyuki
Department of General Surgical Science, Graduate School of Medicine, Gunma University, Maebashi, Japan.
Department of General Surgical Science, Graduate School of Medicine, Gunma University, Maebashi, Japan
Anticancer Res. 2017 Mar;37(3):1043-1048. doi: 10.21873/anticanres.11415.
BACKGROUND/AIM: We detected microRNA-7 (miR-7) as being specific for oesophageal squamous cell carcinoma (ESCC) by using database analysis. However, the significance of miR-7 in clinical ESCC remains unexplored. This study aimed to clarify the clinicopathological significance of miR-7 in ESCC, and investigate miR-7 function.
Quantitative TaqMan reverse transcription polymerase chain reaction was used to evaluate miR-7 expression in 85 ESCC samples to determine the clinicopathological significance of miR-7 expression. The regulation of proliferation by miR-7 was examined with miR-7 precursor-transfected cells.
The expression of miR-7 in ESCC was higher than that in normal tissues. Low expression levels of miR-7 were associated with poor prognosis. Multivariate analysis indicated that low miR-7 expression was an independent prognostic factor for poor survival. In vitro assays showed miR-7 precursor treatment suppressed the proliferation of ESCC cells.
miR-7 might be a promising prognostic marker and therapeutic target in ESCC.
背景/目的：通过数据库分析，我们检测到微小RNA-7（miR-7）对食管鳞状细胞癌（ESCC）具有特异性。然而，miR-7在临床ESCC中的意义仍未得到探索。本研究旨在阐明miR-7在ESCC中的临床病理意义，并研究miR-7的功能。
采用定量TaqMan逆转录聚合酶链反应评估85例ESCC样本中miR-7的表达，以确定miR-7表达的临床病理意义。用转染了miR-7前体的细胞检测miR-7对增殖的调控作用。
ESCC中miR-7的表达高于正常组织。miR-7低表达与预后不良相关。多因素分析表明，miR-7低表达是生存不良的独立预后因素。体外实验显示，miR-7前体处理可抑制ESCC细胞的增殖。
miR-7可能是ESCC中有前景的预后标志物和治疗靶点。
