Hagio Kotaro, Furuya Shinji, Nakamura Jiro, Maruyama Suguru, Shiraishi Kensuke, Shimizu Hiroki, Akaike Hidenori, Hosomura Naohiro, Kawaguchi Yoshihiko, Amemiya Hidetake, Kawaida Hiromichi, Sudo Makoto, Inoue Shingo, Kono Hiroshi, Ichikawa Daisuke
First Department of Surgery, Faculty of Medicine University of Yamanashi, Yamanashi, Japan.
First Department of Surgery, Faculty of Medicine University of Yamanashi, Yamanashi, Japan
Anticancer Res. 2019 Feb;39(2):557-565. doi: 10.21873/anticanres.13148.
BACKGROUND/AIM: Basaloid squamous cell carcinoma of the oesophagus (BSCCE) is a variant of oesophageal squamous cell carcinoma (ESCC), that has a more aggressive biological behaviour than that of typical ESCC. miR-3687 has been previously identified to be highly expressed in BSCCE. This study aimed to evaluate the prognostic impact of miR-3687 in ESCC and determine the role of miR-3687 in ESCC motility.
miR-3687 expression in human ESCC cell lines and in primary tumour samples obtained from patients with ESCC who underwent esophagectomy were analyzed via real-time polymerase chain reaction (PCR). Knockdown and over-expression experiments were conducted with miR-3687 siRNA and miRNA mimic, and the effect on cell proliferation, migration, and invasion was analyzed.
A total of 92 samples were analyzed. High miR-3687 expression was correlated with poor prognosis. miR-3687 upregulation promoted cell migration and invasion.
miR-3687 expression negatively impacts the prognosis of patients with ESCC.
背景/目的：食管基底样鳞状细胞癌（BSCCE）是食管鳞状细胞癌（ESCC）的一种变体，其生物学行为比典型的ESCC更具侵袭性。先前已确定miR-3687在BSCCE中高表达。本研究旨在评估miR-3687对ESCC预后的影响，并确定miR-3687在ESCC运动中的作用。
通过实时聚合酶链反应（PCR）分析miR-3687在人ESCC细胞系以及接受食管切除术的ESCC患者的原发性肿瘤样本中的表达。用miR-3687 siRNA和miRNA模拟物进行敲低和过表达实验，并分析其对细胞增殖、迁移和侵袭的影响。
共分析了92个样本。miR-3687高表达与预后不良相关。miR-3687上调促进细胞迁移和侵袭。
miR-3687表达对ESCC患者的预后有负面影响。
