组蛋白H3和H4信使核糖核酸在玉米中是多聚腺苷酸化的。

Chaubet N, Chaboute M E, Clément B, Ehling M, Philipps G, Gigot C

Institut de Biologie Moléculaire des Plantes, Strasbourg, France.

Nucleic Acids Res. 1988 Feb 25;16(4):1295-304. doi: 10.1093/nar/16.4.1295.

Northern blot analysis revealed that the histone H3 and H4 mRNAs are of unusual large size in germinating maize embryos. S1-mapping experiments show that the 3'-untranslated regions of the mRNAs transcribed from 3 H3 and 2 H4 maize genes previously described are much longer than in the non-polyadenylated histone mRNAs which represent a major class in animals. Moreover, oligo d(T) cellulose fractionation of RNAs isolated at different developmental stages indicates that more than 99% of the maize H3 and H4 mRNAs are polyadenylated. A putative polyadenylation signal is present in all five genes 17 to 27 nucleotides before the 3'-ends of the mRNAs.

Northern印迹分析表明，在萌发的玉米胚中，组蛋白H3和H4的mRNA具有异常大的尺寸。S1作图实验表明，先前描述的3个H3和2个H4玉米基因转录的mRNA的3'非翻译区比动物中代表主要类别的非多聚腺苷酸化组蛋白mRNA长得多。此外，对不同发育阶段分离的RNA进行寡聚d(T)纤维素分级分离表明，超过99%的玉米H3和H4 mRNA是多聚腺苷酸化的。在所有5个基因中，mRNA的3'末端前17至27个核苷酸处存在一个假定的多聚腺苷酸化信号。