Marks M D, Lindell J S, Larkins B A
J Biol Chem. 1985 Dec 25;260(30):16451-9.
A comparison of the DNA and protein sequences of a group of zein cDNA clones reveals that they share extensive sequence homology and probably originated from a common ancestral gene. A comparison of clones corresponding to Mr 22,000 polypeptides shows they are 92% homologous, while five clones corresponding to the Mr 19,000 zeins vary in homology from 75 to 95%. The clones corresponding to the Mr 22,000 proteins are 60-65% homologous to clones encoding the Mr 19,000 zein proteins. A clone corresponding to the Mr 15,000 zein has little homology to either the Mr 22,000 or 19,000 zeins. Clones corresponding to both the Mr 22,000 and 19,000 zeins have two putative polyadenylation signals. S1 nuclease mapping indicates that the first polyadenylation signal following the stop codon is utilized by the Mr 22,000 sequences, while primarily the second polyadenylation signal is utilized by the Mr 19,000 sequences.
一组玉米醇溶蛋白cDNA克隆的DNA和蛋白质序列比较显示，它们具有广泛的序列同源性，可能起源于一个共同的祖先基因。对应于22,000道尔顿多肽的克隆比较表明，它们的同源性为92%，而对应于19,000道尔顿玉米醇溶蛋白的五个克隆的同源性在75%至95%之间变化。对应于22,000道尔顿蛋白质的克隆与编码19,000道尔顿玉米醇溶蛋白的克隆的同源性为60 - 65%。对应于15,000道尔顿玉米醇溶蛋白的一个克隆与22,000道尔顿或19,000道尔顿的玉米醇溶蛋白几乎没有同源性。对应于22,000道尔顿和19,000道尔顿玉米醇溶蛋白的克隆都有两个推定的聚腺苷酸化信号。S1核酸酶图谱分析表明，22,000道尔顿序列利用终止密码子后的第一个聚腺苷酸化信号，而19,000道尔顿序列主要利用第二个聚腺苷酸化信号。
