Bima Abdulhadi, Pezic Angela, Sun Cong, Cameron Fergus J, Rodda Christine, van der Mei Ingrid, Chiaroni-Clarke Rachel, Dwyer Terence, Kemp Andrew, Qu Jun, Carlin John, Ellis Justine A, Ponsonby Anne-Louise
Murdoch Childrens Research Institute, Melbourne, Victoria.
Department of Endocrinology, Sunshine Hospital, University of Melbourne, Melbourne, Victoria.
J Pediatr Endocrinol Metab. 2017 May 1;30(5):531-541. doi: 10.1515/jpem-2016-0088.
Vitamin D deficiency has been associated with adverse health outcomes. We examined genetic and environmental determinants of serum 25(OH)D3 and 1,25(OH)2D3 in childhood.
The study sample consisted of 322 healthy Australian children (predominantly Caucasians) who provided a venous blood sample. A parental interview was conducted and skin phototype and anthropometry measures were assessed. Concentrations of 25(OH)D3 and 1,25(OH)2D3 were measured by selective solid-phase extraction-capillary liquid chromatography-tandem mass spectrometry. These concentrations were deseasonalised where relevant to remove the effect of month of sampling.
Deseasonalised log 25(OH)D3 and 1,25(OH)2D3 concentrations were only moderately correlated (r=0.42, p<0.001). The following predicted both 25(OH)D3 and 1,25(OH)2D3: UVR 6 weeks before the interview, natural skin and eye colour, height and vitamin D allelic metabolism score. The following predicted 25(OH)D3 only: lifetime sunburns and vitamin D allelic synthesis score. Overall, 43.5% and 25.6% of variation in 25(OH)D3 and 1,25(OH)2D3 could be explained. After accounting for 25(OH)D3 concentrations, higher UVR 6 weeks before the interview and vitamin D allelic metabolism score further predicted 1,25(OH)2D3 concentrations.
Environmental factors and genetic factors contributed to both vitamin D metabolite concentrations. The intriguing finding that the higher ambient UVR contributed to higher 1,25(OH)2D3 after accounting for 25(OH)D3 concentrations requires further evaluation.
维生素D缺乏与不良健康结局相关。我们研究了儿童血清25(OH)D3和1,25(OH)2D3的遗传和环境决定因素。
研究样本包括322名健康的澳大利亚儿童（主要为白种人），他们提供了静脉血样本。进行了家长访谈，并评估了皮肤光型和人体测量指标。采用选择性固相萃取-毛细管液相色谱-串联质谱法测定25(OH)D3和1,25(OH)2D3的浓度。在相关情况下对这些浓度进行去季节化处理，以消除采样月份的影响。
去季节化后的log 25(OH)D3和1,25(OH)2D3浓度仅呈中度相关（r = 0.42，p < 0.001）。以下因素可同时预测25(OH)D3和1,25(OH)2D3：访谈前6周的紫外线辐射（UVR）、自然肤色和眼睛颜色、身高以及维生素D等位基因代谢评分。以下因素仅可预测25(OH)D3：终生晒伤次数和维生素D等位基因合成评分。总体而言，25(OH)D3和1,25(OH)2D3变异的43.5%和25.6%可得到解释。在考虑25(OH)D3浓度后，访谈前6周较高的UVR和维生素D等位基因代谢评分进一步预测了1,25(OH)2D3浓度。
环境因素和遗传因素均对维生素D代谢物浓度有影响。在考虑25(OH)D3浓度后，较高的环境UVR导致较高的1,25(OH)2D3这一有趣发现需要进一步评估。
