Hanson Corrine, Anderson-Berry Ann, Lyden Elizabeth, Kaufmann Martin, Wu Amy, Elliott Elizabeth, Lee Jae-In, Jones Glenville
*College of Allied Health Professions, Medical Nutrition Education, 984045 Nebraska Medical Center †Pediatrics, 981205 Nebraska Medical Center ‡College of Public Health, 984375 Nebraska Medical Center, University of Nebraska Medical Center, Omaha §Department of Biomedical and Molecular Sciences, Queen's University, Kingston, ON, Canada.
J Pediatr Gastroenterol Nutr. 2016 Mar;62(3):486-90. doi: 10.1097/MPG.0000000000001001.
Metabolites of vitamin D in maternal-neonatal dyads remain relatively unexplored. The goal of this study was to evaluate concentrations of 25(OH)D3, 24,25(OH)2D3, and 3-epi-25(OH)D3 in maternal-infant pairs at delivery.
Serum samples of maternal and infant cord blood were collected on 131 mother-infant pairs at delivery. Vitamin D metabolites were analyzed in triplicate using liquid chromatography-tandem mass spectrometry. Statistical analysis was conducted using the Fisher exact test, Wilcoxon rank sum test, and Spearman correlation coefficients.
Mean 25(OH)D3 concentrations in maternal and cord blood were 32.9 and 18.5 ng/mL, respectively; mean maternal and cord 24,25(OH)2D3 were 2.0 versus 1.1 ng/mL, respectively. Absolute concentrations of 3-epi-25(OH)D3 were similar in maternal and cord samples (2.4 vs 2.2 ng/mL), whereas the proportion of the total 25(OH)D as the 3-epimer was 6.5% in maternal samples and 10.5% in cord samples. This suggests that the fetus contributes significantly to 3-epi-25(OH)D3 production. In contrast, the ratio of 25(OH)D3:24,25(OH)2D3 was identical in maternal and cord samples (18.5) suggesting equivalent CYP24A1 activity in mother and fetus. Maternal and cord metabolite levels were highly correlated (r = 0.78, 0.90, 0.89 for 25(OH)D3, 24,25(OH)2D3, and 3-epi-25(OH)D3, respectively, P = 0.001 for all). Serum concentrations of all metabolites were lower in nonwhite infants compared with white infants. Maternal and cord concentrations of 25(OH)D3 were positively associated with birth weight (r = 0.21, P = 0.02; r = 0.25, P = 0.003, respectively).
This data suggests that although maternal and cord concentrations of vitamin D metabolites are highly correlated, regulation of specific vitamin D metabolites in the mother and the neonate may be mediated independently.
母婴二元组中维生素D的代谢产物仍相对未被充分研究。本研究的目的是评估分娩时母婴对中25(OH)D3、24,25(OH)2D3和3-表-25(OH)D3的浓度。
在131对母婴分娩时采集母血和婴儿脐带血样本。使用液相色谱-串联质谱法对维生素D代谢产物进行一式三份分析。采用Fisher精确检验、Wilcoxon秩和检验和Spearman相关系数进行统计分析。
母血和脐带血中25(OH)D3的平均浓度分别为32.9和18.5 ng/mL；母血和脐带血中24,25(OH)2D3的平均浓度分别为2.0和1.1 ng/mL。母血和脐带血样本中3-表-25(OH)D3的绝对浓度相似（2.4对2.2 ng/mL），而作为3-表异构体的总25(OH)D的比例在母血样本中为6.5%，在脐带血样本中为10.5%。这表明胎儿对3-表-25(OH)D3的产生有显著贡献。相比之下，母血和脐带血样本中25(OH)D3:24,25(OH)2D3的比值相同（18.5），表明母亲和胎儿中CYP24A1活性相当。母血和脐带血代谢物水平高度相关（25(OH)D3、24,25(OH)2D3和3-表-25(OH)D3的r分别为0.78、0.90、0.89，P均为0.001）。与白人婴儿相比，非白人婴儿所有代谢物的血清浓度较低。母血和脐带血中25(OH)D3的浓度与出生体重呈正相关（r分别为0.21，P = 0.02；r = 0.25，P = 0.003）。
这些数据表明，尽管母婴维生素D代谢物浓度高度相关，但母亲和新生儿中特定维生素D代谢物的调节可能是独立介导的。
