Brignone Desideria, Radmann Pia, Behr Jürgen, Vogel Rudi F
Lehrstuhl für Technische Mikrobiologie, Technische Universität München, Freising, Germany.
Bavarian Center for Biomolecular Mass Spectrometry, Technische Universität München, Gregor-Mendel Str. 4, 85354, Freising, Germany.
Arch Microbiol. 2017 Aug;199(6):853-862. doi: 10.1007/s00203-017-1352-7. Epub 2017 Mar 18.
Single so-called booster substances were added to the fermentation medium of the probiotic strain Lactobacillus (L.) paracasei ssp. paracasei F19 to enhance its growth. A wide screening was carried out in microtiter plates and a statistical analysis of the growth parameters was performed. CFU counts were used to correlate the increase in OD with the increase in viable cell number. Sodium ascorbate, sodium pyruvate, manganese sulfate and cysteine had a remarkable boosting effect on the growth of L. paracasei F19. Three of the boosters increased the growth rate of the strain and led to a higher cell density and biomass yield in laboratory conditions. Cysteine significantly shortened the lag phase, therefore reducing the fermentation times. The boosters were tested on four additional Lactobacillus species and their growth boosting activity was retained. To investigate whether the growth boosters could improve the tolerance of L. paracasei F19 to the adverse condition in the GI tract, additional tests were performed. Sodium ascorbate and sodium pyruvate exerted a certain antioxidant effect, as they improved the tolerance of L. paracasei F19 to HO. Sodium ascorbate enhanced the growth of the strain in low pH.
将单一的所谓促进物质添加到益生菌副干酪乳杆菌亚种副干酪乳杆菌F19的发酵培养基中，以促进其生长。在微量滴定板中进行了广泛筛选，并对生长参数进行了统计分析。使用菌落形成单位（CFU）计数来关联光密度（OD）的增加与活细胞数的增加。抗坏血酸钠、丙酮酸钠、硫酸锰和半胱氨酸对副干酪乳杆菌F19的生长具有显著的促进作用。其中三种促进剂提高了该菌株的生长速率，并在实验室条件下导致更高的细胞密度和生物量产量。半胱氨酸显著缩短了延滞期，从而缩短了发酵时间。对另外四种乳酸杆菌进行了促进剂测试，它们的生长促进活性得以保留。为了研究生长促进剂是否能提高副干酪乳杆菌F19对胃肠道不利条件的耐受性，进行了额外的测试。抗坏血酸钠和丙酮酸钠发挥了一定的抗氧化作用，因为它们提高了副干酪乳杆菌F19对过氧化氢的耐受性。抗坏血酸钠在低pH值条件下促进了该菌株的生长。
