Oliveira Layssa M, Orílio Anelise F, Inoue-Nagata Alice K, Nagata Tatsuya, Blawid Rosana
Department Cell Biology, University of Brasília, 70910-900, Brasília, DF, Brazil.
Embrapa Vegetables, Caixa Postal 218, 70275-970, Brasília, DF, Brazil.
Arch Virol. 2017 Jul;162(7):2141-2144. doi: 10.1007/s00705-017-3326-0. Epub 2017 Mar 18.
High throughput sequencing (HTS) is a very powerful tool for detecting and discovering novel viral-like sequences without prior knowledge of the sequence. Here we describe the complete genome of a new vitivirus-like sequence that was found in arracacha (Arracacia xanthorrhiza) plants using HTS technology. The complete genome sequence was validated by Sanger sequencing. The genomic organization of the new putative vitivirus resembles that of grapevine virus B (GVB) and grapevine virus D (GVD). The putative coat protein showed 41 to 49% identity with similar proteins of known vitiviruses, while the RNA-dependent RNA polymerase shared 52 to 55% identity with those encoded by grapevine vitiviruses. Based on the demarcation criteria for the genus Vitivirus, the virus described in this work, provisionally named as "Arracacha virus V", represents a novel species in this taxon.
高通量测序（HTS）是一种非常强大的工具，可用于在无需预先了解序列的情况下检测和发现新的类病毒序列。在此，我们描述了一种利用HTS技术在阿拉卡查（Arracacia xanthorrhiza）植物中发现的新类葡萄病毒序列的完整基因组。完整基因组序列通过桑格测序进行了验证。新的假定类葡萄病毒的基因组结构类似于葡萄病毒B（GVB）和葡萄病毒D（GVD）。假定的外壳蛋白与已知类葡萄病毒的相似蛋白具有41%至49%的同一性，而RNA依赖的RNA聚合酶与葡萄类葡萄病毒编码的那些聚合酶具有52%至55%的同一性。根据类葡萄病毒属的分类标准，本研究中描述的病毒，暂命名为“阿拉卡查病毒V”，代表了该分类单元中的一个新物种。
