Gaur Ruchi Singh, Sudan Vikrant, Jaiswal Amit Kumar, Singh Amit, Shanker Daya
Department of Parasitology, College of Veterinary Sciences and Animal Husbandry, U. P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura, 281001 India.
J Parasit Dis. 2017 Mar;41(1):51-54. doi: 10.1007/s12639-016-0748-7. Epub 2016 Mar 16.
Trypanosomosis or Surra can rightly be attributed as the most economically important vector-borne haemoprotozoan disease encountering India. Surra infected chronic cases show almost similar types of signs and symptoms often confusing it with other haemoprotozoan infections, thereby, making it prerequisite for the development of aspecific and sensitive technique for its detection in susceptible animals. Blood microscopy and serology suffers from the hands of lack of sensitivity and specificity thereby leaving molecular detection techniques as one of the promising alternative. Alongside, there is utmost need for exploring of new molecular gene targets for the development of a putative alternative for diagnosis and immunoprophylaxsis. The present communication describes the identification and amplification of oligopeptidase B, cysteine protease and variable surface glycoprotein genes of so as to exploit them in future as potential candidates for immune protection and/or molecular detection.
锥虫病或苏拉病无疑可被视为印度面临的最具经济重要性的媒介传播血液原虫病。感染苏拉病的慢性病例表现出几乎相似类型的体征和症状，常常使其与其他血液原虫感染相混淆，因此，开发一种特异且灵敏的技术用于在易感动物中检测该病成为必要条件。血液显微镜检查和血清学检测存在灵敏度和特异性不足的问题，从而使分子检测技术成为一种有前景的替代方法。此外，迫切需要探索新的分子基因靶点，以开发用于诊断和免疫预防的假定替代方法。本通讯描述了寡肽酶B、半胱氨酸蛋白酶和可变表面糖蛋白基因的鉴定和扩增，以便将来将它们用作免疫保护和/或分子检测的潜在候选物。
