Sudan Vikrant, Jaiswal Amit Kumar, Shanker Daya, Verma Amit Kumar
College of Veterinary Science and Animal Husbandry, U.P. Pandit Deen Dayal Upadhyaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura, 281001, India.
Trop Anim Health Prod. 2017 Dec;49(8):1793-1796. doi: 10.1007/s11250-017-1384-7. Epub 2017 Aug 22.
Rotat 1.2 variant surface glycoprotein (VSG) is considered to be an important VSG expressed in most of the isolates of Trypanosoma evansi. This makes the molecule an important candidate for both molecular- and serological-based detection of surra. There are ample reports of existence of this gene in isolates from cattle, buffalo, and camel across the world. Of late, there are reports of its absence from a fewer isolates of T. evansi of murine and wildlife origin. Search of literature revealed no reports from horses. The present communication presents the first report of molecular cloning and characterization of Rotat 1.2 VSG from horse isolate of T. evansi from semi-arid region of India. Alongside, the gene was compared with various other isolates across the world. Interestingly, the isolate was found to be closer to camel isolates from Egypt than the other known isolates from India and Kenya.
Rotat 1.2变异表面糖蛋白（VSG）被认为是在大多数伊氏锥虫分离株中表达的一种重要VSG。这使得该分子成为基于分子和血清学检测苏拉病的重要候选物。世界各地有大量关于该基因存在于牛、水牛和骆驼分离株中的报道。最近，有报道称在少数鼠源和野生动物源的伊氏锥虫分离株中不存在该基因。文献检索未发现来自马的相关报道。本通讯首次报道了从印度半干旱地区伊氏锥虫马分离株中分子克隆和鉴定Rotat 1.2 VSG。同时，将该基因与世界各地的其他各种分离株进行了比较。有趣的是，发现该分离株比来自印度和肯尼亚的其他已知分离株更接近埃及的骆驼分离株。
