使用酶联免疫吸附测定法（ELISA）和斑点酶免疫测定法（Dot-EIA）基于抗原检测水牛的囊型包虫病。
Antigen based detection of cystic echinococcosis in buffaloes using ELISA and Dot-EIA.
作者信息
Sangaran A, Bino Sundar S T, Latha Bhaskaran Ravi
机构信息
Department of Veterinary Parasitology, Madras Veterinary College, Tamilnadu Veterinary and Animal Sciences University, Chennai, 600 007 India.
出版信息
J Parasit Dis. 2017 Mar;41(1):128-130. doi: 10.1007/s12639-016-0762-9. Epub 2016 Apr 4.
Cystic echinococcosis is caused by the larval stage of the dog tapeworm, . The disease is recognized as one of the world's major zoonoses affecting human beings and domestic animals apart from its economic and public health importance. Development of the cysts in the intermediate host such as buffaloes occurs in the lungs, liver and other organs. In this study, detection of circulating antigen in the diagnosis of cystic echinococcosis in buffaloes was done using enzyme linked immunosorbent assay and Dot-Enzyme immunoassay (Dot-EIA). The sensitivity and specificity were determined as 89 and 92 % respectively, whereas those of Dot-EIA were determined as 94 and 96 %.
囊型包虫病由犬绦虫的幼虫阶段引起。除了其经济和公共卫生重要性外，该疾病被认为是影响人类和家畜的世界主要人畜共患病之一。中间宿主如水牛体内囊肿的发育发生在肺、肝脏和其他器官。在本研究中，使用酶联免疫吸附测定法和斑点酶免疫测定法（Dot-EIA）对水牛囊型包虫病诊断中的循环抗原进行检测。酶联免疫吸附测定法的敏感性和特异性分别确定为89%和92%，而斑点酶免疫测定法的敏感性和特异性分别确定为94%和96%。
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