Moosa R A, Abdel-Hafez S K
Department of Biological Sciences, Yarmouk University, Irbid, Jordan.
Parasitol Res. 1994;80(8):664-71. doi: 10.1007/BF00932950.
A total of 2182 serum samples from 38 patients with surgically confirmed unilocular hydatidosis, 19 clinically assessed patients, 15 patients with parasitic infections other than hydatidosis, 104 hospital outpatients, and 2006 normal Jordanians were serodiagnosed for the presence of IgG antibodies against hydatid fluid, circulating immune complexes (CIC), and/or hydatid circulating antigen (CA). Anti-hydatid IgG antibodies were detected in the sera of 77.4% of patients with hydatid disease and persist for very long periods postsurgery. As many as 54.1% of patients with hydatidosis had positive levels of CIC, and 16.1% had circulating antigen in their sera. The search for circulating antigen and CIC decreased the number of false-negative hydatid cases from seven to three, and the combined sensitivity of the assays thus increased from 77.4% to 90.3%. Using the immunoblot technique, 16- and < 14.4-kDa Echinococcus granulosus-specific bands were detected in sera from 54.1% and 61.5% of patients with hydatid disease who were tested before and after surgery, respectively. The seropositivity rate for anti-hydatid IgG antibodies was 2.4% for the general Jordanian population and 5.8% for hospital outpatients.
对38例经手术确诊为单房性包虫病的患者、19例经临床评估的患者、15例患有除包虫病之外寄生虫感染的患者、104例医院门诊患者以及2006名健康约旦人的总共2182份血清样本进行了血清学诊断，以检测是否存在抗包虫液IgG抗体、循环免疫复合物（CIC）和/或包虫循环抗原（CA）。77.4%的包虫病患者血清中检测到抗包虫IgG抗体，且术后很长时间内持续存在。多达54.1%的包虫病患者CIC水平呈阳性，16.1%的患者血清中有循环抗原。检测循环抗原和CIC使包虫病假阴性病例数从7例减少到3例，因此检测方法的联合敏感性从77.4%提高到90.3%。采用免疫印迹技术，术前和术后接受检测的包虫病患者血清中，分别有54.1%和61.5%检测到16 kDa和<14.4 kDa的细粒棘球绦虫特异性条带。约旦普通人群中抗包虫IgG抗体的血清阳性率为2.4%，医院门诊患者为5.8%。
