埃及上埃及蜱虫感染牛群中牛巴贝斯虫病（）诊断的传统方法与分子方法比较
Comparison between conventional and molecular methods for diagnosis of bovine babesiosis () in tick infested cattle in upper Egypt.
作者信息
Al-Hosary Amira A T
机构信息
Department of Animal Medicine Infectious Diseases, Faculty of Veterinary Medicine, Assiut University, Assiut, 71526 Egypt.
出版信息
J Parasit Dis. 2017 Mar;41(1):243-246. doi: 10.1007/s12639-016-0785-2. Epub 2016 Jun 22.
Abstract
Ticks and tick-borne diseases are the main problems affecting the livestock production in Egypt. Bovine babesiosis has adverse effects on the animal health and production. A comparison of Giemsa stained blood smears, polymerase chain reaction (PCR) and nested PCR (nPCR) assays for detection of infection in Egyptian Baladi cattle () in reference to reverse line blot was carried out. The sensitivity of PCR and nested PCR (nPCR) assays were 65 and 100 % respectively. Giemsa stained blood smears showed the lowest sensitivity (30 %). According to these results using of PCR and nPCR target for , [BBOV-IV005650 (BV5650)] gene are suitable for diagnosis of infection. The 18Ss rRNA partial sequence confirmed that all the positive samples were and all of them were deposited in the GenBank databases (Accession No: KM455548, KM455549 and KM455550).
摘要
蜱虫及蜱传疾病是影响埃及畜牧业生产的主要问题。牛巴贝斯虫病对动物健康和生产有不利影响。针对埃及巴莱迪牛（）感染情况，开展了吉姆萨染色血涂片、聚合酶链反应（PCR）和巢式PCR（nPCR）检测方法与反向线印迹法的比较。PCR和巢式PCR（nPCR）检测方法的灵敏度分别为65%和100%。吉姆萨染色血涂片显示出最低的灵敏度（30%）。根据这些结果，针对[BBOV-IV005650（BV5650）]基因使用PCR和nPCR靶点适用于诊断感染。18Ss rRNA部分序列证实所有阳性样本均为，且所有样本均已存入GenBank数据库（登录号：KM455548、KM455549和KM455550）。
相似文献
1
Comparison between conventional and molecular methods for diagnosis of bovine babesiosis () in tick infested cattle in upper Egypt.埃及上埃及蜱虫感染牛群中牛巴贝斯虫病（）诊断的传统方法与分子方法比较
J Parasit Dis. 2017 Mar;41(1):243-246. doi: 10.1007/s12639-016-0785-2. Epub 2016 Jun 22.
2
Development and evaluation of two nested PCR assays for the detection of Babesia bovis from cattle blood.牛巴贝斯虫巢式 PCR 检测方法的建立与评价
Vet Parasitol. 2010 Aug 27;172(1-2):65-70. doi: 10.1016/j.vetpar.2010.04.011. Epub 2010 Apr 18.
3
International interlaboratory validation of a nested PCR for molecular detection of Babesia bovis and Babesia bigemina, causative agents of bovine babesiosis.牛巴贝斯虫和双芽巴贝斯虫的巢式 PCR 分子检测的国际实验室间验证。
Vet Parasitol. 2022 Apr;304:109686. doi: 10.1016/j.vetpar.2022.109686. Epub 2022 Mar 3.
4
Serological and molecular diagnostic surveys combined with examining hematological profiles suggests increased levels of infection and hematological response of cattle to babesiosis infections compared to native buffaloes in Egypt.血清学和分子诊断调查结合血液学特征检查表明，与埃及本地水牛相比，牛对巴贝斯虫病感染的感染水平和血液学反应有所增加。
Parasit Vectors. 2015 Jun 12;8:319. doi: 10.1186/s13071-015-0928-9.
5
Molecular and serological detection of Babesia bovis- and Babesia bigemina-infection in bovines and water buffaloes raised jointly in an endemic field.在地方病流行地区共同饲养的牛和水牛中，牛巴贝斯虫和双芽巴贝斯虫感染的分子和血清学检测
Vet Parasitol. 2016 Feb 15;217:101-7. doi: 10.1016/j.vetpar.2015.12.030. Epub 2016 Jan 2.
6
Molecular surveillance and phylogenetic traits of Babesia bigemina and Babesia bovis in cattle (Bos taurus) and water buffaloes (Bubalus bubalis) from Colombia.哥伦比亚牛（Bos taurus）和水牛（Bubalus bubalis）中的双芽巴贝斯虫和牛巴贝斯虫的分子监测和系统发育特征。
Parasit Vectors. 2018 Sep 12;11(1):510. doi: 10.1186/s13071-018-3091-2.
7
High co-infection rates of Babesia bovis, Babesia bigemina, and Anaplasma marginale in water buffalo in Western Cuba.古巴西部水牛中巴贝斯虫、双芽巴贝斯虫和边缘无形体的高共感染率。
Parasitol Res. 2019 Mar;118(3):955-967. doi: 10.1007/s00436-018-06194-6. Epub 2019 Jan 28.
8
Development and evaluation of a nested PCR based on spherical body protein 2 gene for the diagnosis of Babesia bovis infection.基于球形蛋白 2 基因的巢式 PCR 的开发与评估用于诊断牛巴贝斯虫感染。
Vet Parasitol. 2010 Apr 19;169(1-2):45-50. doi: 10.1016/j.vetpar.2009.12.013. Epub 2009 Dec 22.
9
Molecular and serological prevalence of Babesia bovis and Babesia bigemina in cattle from central region of Syria.叙利亚中部地区牛巴贝斯虫和双芽巴贝斯虫的分子和血清流行情况。
Vet Parasitol. 2012 Jun 8;187(1-2):307-11. doi: 10.1016/j.vetpar.2011.12.038. Epub 2012 Jan 5.
10
Molecular investigation of tick-borne infections in cattle from Xinjiang Uygur Autonomous Region, China.中国新疆维吾尔自治区牛蜱传感染的分子调查。
Parasitol Int. 2020 Feb;74:101925. doi: 10.1016/j.parint.2019.05.003. Epub 2019 May 8.
引用本文的文献
1
First detection of spp. in and molecular characterization of piroplasmids in southern Egypt.埃及南部首次检测到 spp. 以及梨形虫分子特征分析
Front Vet Sci. 2025 Jun 18;12:1617204. doi: 10.3389/fvets.2025.1617204. eCollection 2025.
2
Isolation and propagation of an Egyptian Theileria annulata infected cell line and evaluation of its use as a vaccine to protect cattle against field challenge.埃及泰勒虫感染细胞系的分离和增殖及其作为疫苗用于保护牛免受田间攻击的评估。
Sci Rep. 2024 Apr 12;14(1):8565. doi: 10.1038/s41598-024-57325-2.
3
Genetic Characterization of the RAP-1A and SBP-4 Genes of Species Infecting Cattle from Selangor, Malaysia, and Ribah, Nigeria.马来西亚雪兰莪州和尼日利亚里巴地区感染牛的物种的RAP - 1A和SBP - 4基因的遗传特征分析
Pathogens. 2024 Mar 13;13(3):247. doi: 10.3390/pathogens13030247.
4
Validation of a multiplex PCR assay to detect spp. and in cattle in Uruguay in the absence of a gold standard test.在没有金标准检测方法的情况下，验证一种多重 PCR 检测方法，以检测乌拉圭牛中的 spp. 和 。
J Vet Diagn Invest. 2021 Jan;33(1):73-79. doi: 10.1177/1040638720975742. Epub 2020 Nov 30.
5
Research on Integrated Management for Cattle Fever Ticks and Bovine Babesiosis in the United States and Mexico: Current Status and Opportunities for Binational Coordination.美国和墨西哥牛蜱热与牛巴贝斯虫病综合管理研究：双边协调的现状与机遇
Pathogens. 2020 Oct 23;9(11):871. doi: 10.3390/pathogens9110871.
6
Epidemiology and genotyping of Anaplasma marginale and co-infection with piroplasms and other Anaplasmataceae in cattle and buffaloes from Egypt.埃及牛和水牛中的边缘无浆体的流行病学和基因分型以及与梨形虫和其他无浆体科的共感染。
Parasit Vectors. 2020 Sep 29;13(1):495. doi: 10.1186/s13071-020-04372-z.
7
Comparative evaluation of DNA extraction kit, matrix sample and qPCR assays for bovine babesiosis monitoring.用于牛巴贝斯虫病监测的DNA提取试剂盒、基质样本和定量聚合酶链反应检测方法的比较评估
Mol Biol Rep. 2018 Dec;45(6):2671-2680. doi: 10.1007/s11033-018-4436-9. Epub 2018 Oct 25.
本文引用的文献
1
Molecular biological identification of Babesia, Theileria, and Anaplasma species in cattle in Egypt using PCR assays, gene sequence analysis and a novel DNA microarray.使用聚合酶链反应（PCR）检测、基因序列分析及新型DNA微阵列技术对埃及牛体内巴贝斯虫、泰勒虫和无形体属物种进行分子生物学鉴定
Vet Parasitol. 2015 Jan 30;207(3-4):329-34. doi: 10.1016/j.vetpar.2014.12.025. Epub 2014 Dec 30.
2
An epidemiological survey of bovine Babesia and Theileria parasites in cattle, buffaloes, and sheep in Egypt.埃及牛、水牛和绵羊中巴贝斯虫属和泰勒虫属寄生虫的流行病学调查。
Parasitol Int. 2015 Feb;64(1):79-85. doi: 10.1016/j.parint.2014.10.002. Epub 2014 Oct 14.
3
Molecular and serological prevalence of Babesia bigemina and Babesia bovis in cattle and water buffalos under small-scale dairy farming in Beheira and Faiyum Provinces, Egypt.埃及贝尼苏韦夫省和法尤姆省小规模奶牛养殖条件下，牛和水牛巴贝西虫病和双芽巴贝斯虫病的分子和血清流行情况。
Vet Parasitol. 2013 Nov 15;198(1-2):187-92. doi: 10.1016/j.vetpar.2013.08.028. Epub 2013 Sep 6.
4
Current advances in detection and treatment of babesiosis.巴贝斯虫病的检测和治疗的最新进展。
Curr Med Chem. 2012;19(10):1504-18. doi: 10.2174/092986712799828355.
5
Development and evaluation of two nested PCR assays for the detection of Babesia bovis from cattle blood.牛巴贝斯虫巢式 PCR 检测方法的建立与评价
Vet Parasitol. 2010 Aug 27;172(1-2):65-70. doi: 10.1016/j.vetpar.2010.04.011. Epub 2010 Apr 18.
6
7
Development and evaluation of a nested PCR based on spherical body protein 2 gene for the diagnosis of Babesia bovis infection.基于球形蛋白 2 基因的巢式 PCR 的开发与评估用于诊断牛巴贝斯虫感染。
Vet Parasitol. 2010 Apr 19;169(1-2):45-50. doi: 10.1016/j.vetpar.2009.12.013. Epub 2009 Dec 22.
8
Detection of bovine babesiosis in Mozambique by a novel seminested hot-start PCR method.采用新型半巢式热启动聚合酶链反应方法检测莫桑比克牛巴贝斯虫病
Vet Parasitol. 2008 May 31;153(3-4):225-30. doi: 10.1016/j.vetpar.2008.01.037. Epub 2008 Feb 6.
9
Comparison of different direct diagnostic methods to identify Babesia bovis and Babesia bigemina in animals vaccinated with live attenuated parasites.比较不同直接诊断方法以鉴定接种减毒活寄生虫疫苗的动物中的牛巴贝斯虫和双芽巴贝斯虫。
Vet Parasitol. 2006 Jun 30;139(1-3):231-6. doi: 10.1016/j.vetpar.2006.02.034. Epub 2006 Mar 31.
10
PCR-based detection of Babesia bovis and Babesia bigemina in their natural host Boophilus microplus and cattle.基于聚合酶链反应在微小牛蜱及牛等自然宿主中检测牛巴贝斯虫和双芽巴贝斯虫。
Int J Parasitol. 2005 Jan;35(1):105-11. doi: 10.1016/j.ijpara.2004.09.002.
Zotero 插件