LB肉汤-冻干兔血清（LLR）作为一种用于培养……前鞭毛体的新型适宜培养基。 （注：原文句末不完整，翻译只能到此为止）
LB broth-lyophilized Rabbit serum (LLR) as a new and suitable culture medium for cultivation of promastigotes of .
作者信息
Nasiri Vahid, Dalimi Abdolhossein, Ghaffarifar Fatemeh
机构信息
Department of Parasitology, Razi Vaccine and Serum Research Institute, Alborz, Iran.
Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
出版信息
J Parasit Dis. 2017 Mar;41(1):247-251. doi: 10.1007/s12639-016-0786-1. Epub 2016 May 26.
Fetal calf serum is the major part and the most expensive ingredient of the culture media. Here, the efficacy of the LB broth-lyophilized Rabbit serum medium (LLR) was evaluated in cultivation of . Conventional Luria-Bertani (LB) broth medium was prepared and autoclaved for 15 min at 121 °C and then lyophilized Rabbit serum was added at the 1, 2.5, 5 and 10 % final concentrations. The efficacy of medium was evaluated by assessing the growth ability and replication pattern of the promastigotes of . According to our finding, the LLR medium with 5-10 % lyophilized Rabbit serum supported the growth of the parasites and can be used for cultivation of Leishmanian parasites with acceptable In vivo infectivity for research purpose. The ability of the parasites to survive and proliferating in the presence of lyophilized Rabbit serum indicating that this serum is a good nutritional source. This study opens a new way to make low-cost medium that could be used in cultivation of Leishmanian parasites.
胎牛血清是培养基的主要成分，也是最昂贵的成分。在此，评估了LB肉汤-冻干兔血清培养基（LLR）在培养……中的功效。制备常规的Luria-Bertani（LB）肉汤培养基，并在121℃下高压灭菌15分钟，然后分别以1%、2.5%、5%和10%的终浓度添加冻干兔血清。通过评估……前鞭毛体的生长能力和增殖模式来评价培养基的功效。根据我们的研究结果，含有5%-10%冻干兔血清的LLR培养基支持寄生虫生长，可用于培养具有可接受的体内感染性的利什曼原虫寄生虫以供研究之用。寄生虫在冻干兔血清存在下存活和增殖的能力表明这种血清是一种良好的营养来源。本研究为制备可用于培养利什曼原虫寄生虫的低成本培养基开辟了一条新途径。
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